Cell-mediated and humoral immune responses to a virulent plasmid-cured mutant strain of Salmonella enterica serotype gallinarum in broiler chickens

Veterinary Microbiology
Neeraj Rana, Ramesh C Kulshreshtha

Abstract

One-week-old Salmonella-free broiler chicks were subcutaneously immunized and subsequently boosted two weeks later with 2x10(7)cfu 0.5ml(-1) of an 85-kb virulent plasmid-cured spectinomycin-resistant mutant strain (SG9VP(-)Spc(r)) of Salmonella gallinarum 9 (S. enterica serotype gallinarum 9) along with a control group of mock-immunized chickens. The chicks were subcutaneously challenged at 5 weeks of age with 5x10(10)cfu 0.5ml(-1) (5xLD(50)) of wild-type S. gallinarum var. Duisburg (S. enterica serotype gallinarum var. Duisburg). The cellular and humoral immune responses were measured at weekly intervals post-immunization (PI) and post-challenge (PC) using lymphocyte stimulation test (LST), delayed type hypersensitivity (DTH) test, serum tube agglutination test (STAT) and enzyme-linked immunosorbent assay (ELISA). High stimulation indices suggestive of a potent lymphocyte transformation response and high persistent serum IgG titres were recorded in immunized chickens at the termination of the experiment. These findings indicate that the live attenuated mutant vaccine induced a strong cellular and humoral immunity, which may play a role in the protection of fowl typhoid in broiler chickens.

Citations

Jun 30, 2011·Avian Pathology : Journal of the W.V.P.A·Kiku MatsudaJohn Hwa Lee
Jun 1, 2014·Comparative Immunology, Microbiology and Infectious Diseases·Chetan V Jawale, John Hwa Lee
Oct 25, 2016·The Journal of Poultry Science·Kuo-Lung ChenChishih Chu
Nov 3, 2020·Immunity, Inflammation and Disease·Pengtao PanJunlei Yin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.