PMID: 6172086Jan 1, 1981Paper

Cell-mediated immune reactions as probes of sensitization to tumour-associated antigens of methylcholanthrene-induced mouse sarcomas

Archiv für Geschwulstforschung
J BubeníkJ Símová

Abstract

The evaluation of the Field-Caspary macrophage electrophoretic mobility (MEM) assay as a probe of sensitization to tumour-associated antigens was performed in the genetically defined experimental system of methylcholanthrene-induced sarcomas in C57BL10/ScSnPh mice. Six sarcomas carrying noncross-reacting tumour-associated transplantation antigens were examined. For comparison, antigenic specificity of the six sarcomas was simultaneously assessed by the microcytotoxicity assay with 3H-thymidine. Immunization of mice with syngeneic tumour cells as well as sensitization of lymph node cells (LNC) in vitro lead to the generation of cytotoxic lymphocytes. Tumour bearers' LNC were not cytotoxic; when added to preimmunized LNC, the tumour bearers' LNC suppressed cytotoxicity. In contrast to the results obtained with the microcytotoxicity assay, the MEM test gave positive results with LNC from both tumour-bearing and tumour-immunized mice. Whereas some cross-reactions were observed among the fibrosarcomas in the microcytotoxicity assay (6 out of 28 combinations examined), the results of the Field-Caspary MEM test showed the same individually specific pattern of antigenicity as observed in transplantation tests and revealed no cross-reac...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.