Cell-mediated immune response to fractionated products of Actinomyces viscosus cultures

Journal of Periodontology
D W TurnerV J Berzinskas

Abstract

Since bacterial invasion of host tissue has not been shown to occur in the course of periodontitis, many investigators believe that products released from dental plaque microorganisms enter the tissue and elicit a series of host responses leading to the pathological condition. This study, therefore, was designed to explore the possibility that cell-free products isolated from Actinomyces viscosus cultures are able to induce cell-mediated immunological responses in experimental animals. Hartley strain guinea pigs were sensitized with concentrated cell-free products of A. viscosus culture supernatant fluids mixed with Freund complete adjuvant. After 10 to 17 days the animals responded to challenge with this material with skin reactions indicative of delayed-type hypersensitivity. Furthermore, cells isolated from sensitized animals produced mitogenic factor and macrophage migration inhibition factor (MIF) when cultured with the material. The cell-free culture products were fractionated by column chromatography and electrophoresis. Several partially purified components were shown to induce cell-mediated responses in guinea pigs as determined by skin tests and by mitogenic factor and MIF assays. It is apparent that this single micro...Continue Reading

References

Mar 1, 1979·Journal of Periodontology·M E NeidersJ R Natiella
Aug 1, 1976·Infection and Immunity·D W TurnerR W Longton
Jan 1, 1972·Journal of Periodontal Research·H V JordanS Bellack
May 1, 1969·The Journal of the American Dental Association·R J GencoS A Ellison
Mar 1, 1971·Journal of Dental Research· LöH
May 1, 1967·Journal of Periodontology·B DiensteinR K Williams
Apr 1, 1970·Journal of Periodontology·R J Nisengard, E H Beutner
Dec 28, 1964·Annals of the New York Academy of Sciences·L ORNSTEIN
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actinomycosis

Actinomycosis is a rare, chronic, and slowly progressive granulomatous disease caused by filamentous Gram positive anaerobic bacteria from the Actinomycetaceae family (genus Actinomyces). The disease is characterised by the formation of painful abscesses in the mouth, lungs,breast or gastrointestinal tract. Discover the latest research on actinomycosis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.