Cell-mediated immunity is depressed following cardiopulmonary bypass
Abstract
Sequential in vitro lymphocyte function tests in 13 patients undergoing cardiac operation were performed to determine factors that contribute to depressed cell-mediated immunity following operation. Lymphocytes were stimulated with phytohemagglutinin (PHA), pokeweed mitogen, concanavalin A (Con A), and mitomycin-treated, pooled, allogeneic lymphocytes (MLC). Mitogen responses were measured by 3H-labeled thymidine incorporation. Circulating levels of T, B, and Fc-receptor lymphocytes were determined by counting E, EAC, and EA rosettes. Serum cortisol was measured by radioimmunoassay. The T-cell-dependent lymphocyte responses (PHA, Con A, and MLC) were significantly decreased 24 hours after operation, and this was accompanied by a 60% decrease in circulating T-cell levels. The PHA, Con A, and MLC responses, and circulating T-cell levels returned to preoperative values one week following operation. Lymphocyte responses to mitogens remained significantly decreased when the number of T cells in the postoperative cultures were adjusted to preoperative levels. This indicates that the T cells remaining after operation were functionally impaired. We conclude that lymphocyte proliferative responses and antigen recognition are significant...Continue Reading
References
Citations
Related Concepts
Related Feeds
Allogenic & Autologous Therapies
Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.