Cell-mediated immunity to pseudorabies virus: cytolytic effector cells with characteristics of lymphokine-activated killer cells lyse virus-infected and glycoprotein gB- and gC-transfected L14 cells

The Journal of General Virology
T G KimmanA T Bianchi

Abstract

We examined cytolytic cells that lyse pseudorabies virus (PRV)-infected cells in pigs. In vitro stimulation of peripheral blood mononuclear cells from PRV-immune pigs with live PRV generated cells that lysed PRV-infected immortalized B cells. Several lines of evidence indicated a major contribution of non-major histocompatibility complex (MHC)-restricted cytolytic cells, which displayed characteristics of natural killer (NK) or lymphokine-activated killer cells: cytolysis was non-MHC-restricted, depended on CD2+CD4-CD8bright- (or CD2+CD4-CD8dull+) cells, was strongly augmented by in vitro antigenic stimulation and was not limited to virus-infected cells, i.e. the NK cell-susceptible target cell line K562 was also lysed. Cytolytic cells were also generated by in vitro antigenic stimulation with UV-inactivated PRV. Target cells transfected with and stably expressing PRV gB or gC were lysed to the same degree as PRV-infected target cells.

Citations

Feb 27, 1998·Veterinary Immunology and Immunopathology·M G de BruinT G Kimman
Sep 16, 1999·Journal of Biotechnology·A SaalmüllerE Pfaff
Aug 22, 2001·The Veterinary Quarterly·J T van Oirschot
Mar 12, 2010·Clinical and Vaccine Immunology : CVI·Laura C MillerKelly M Lager
May 17, 2000·The Journal of General Virology·T G de BruinA T Bianchi

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