Cell membrane integrity and distinguishing between metabolically active and inactive cells as a means of improving viability PCR

Molecular and Cellular Probes
Francesc CodonyAnna Allué-Guardia

Abstract

Viability PCR uses cell membrane integrity to differentiate live cells from dead. Our new approach improves viability PCR by enabling it to also discriminate between cells with an intact cell membrane and the ability to actively maintain bacterial homeostasis and cells that have an intact membrane but are metabolically inactive.

References

Feb 5, 2005·Applied and Environmental Microbiology·Knut RudiAskild L Holck
Jun 5, 2007·Journal of Microbiological Methods·Andreas NockerAnne K Camper
Sep 4, 2012·Journal of Microbiological Methods·Mariana FittipaldiFrancesc Codony
Nov 21, 2012·Antimicrobial Agents and Chemotherapy·Liliana RodriguesJosé A Aínsa
Jan 22, 2014·Journal of Microbiological Methods·Hui WangXianqin Yang
May 16, 2014·PloS One·Vijaya Bharathi SrinivasanGovindan Rajamohan

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Citations

Sep 18, 2016·Chemosphere·Adriana Chávez-CalderónAntonia Luna-Velasco
Sep 22, 2018·FEMS Microbiology Ecology·Morgane GuéganClaire Valiente Moro
Dec 8, 2019·Current Microbiology·F CodonyG Agustí
Feb 9, 2017·Current Microbiology·Gemma AgustíFrancesc Codony
Dec 29, 2020·Applied and Environmental Microbiology·Wannes Van HolmWim Teughels
Apr 10, 2021·International Journal of Food Microbiology·Amir Saber MaroufJalal Shayegh

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