Cell membrane labeling with fluorescent dyes for the demonstration of cytokine-induced fusion between monocytes and tumor cells

Cytometry
L SpötlJ Möst

Abstract

Fusion between monocytes and tumor cells has been suggested as a cause for tumor metastasis. The aim of the present study was to establish an in vitro fusion model representing the in vivo situation as close as possible. For this purpose fusion between cells was induced by cytokine containing conditioned medium. In order to prove that hybrid formation between tumor cells and monocytes occurs, a two-color-fusion-assay based on membrane labeling with the fluorochromes PKH 2 (green) and PKH 26 (red) was established. These fusion experiments were analyzed by microscopy and, in addition, by flow cytometry. The attempt to induce fusion between monocytes and several tumor cell lines of hematopoietic origin revealed quite diverse results. The most extensive hybrid formations were seen with TALL, a T-lymphocytic tumor line. The monocytic tumor line HL60 and the B-lymphocytic tumor line BL41 also clearly yielded hybrids with monocytes but in smaller numbers. With some other hematopoietic tumor lines no evidence for hybrid formation was detected. These studies indicate that fusion of normal monocytes with certain tumor cells may be induced under conditions that may occur in comparable manner in vivo.

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Citations

May 26, 2006·Antimicrobial Agents and Chemotherapy·Clement MugabeAbdelwahab Omri
Apr 17, 2014·In Vitro Cellular & Developmental Biology. Animal·Miriam NagyovaDasa Cizkova
Dec 29, 2007·Immunological Investigations·Joseph J SkitzkiSharon S Evans
Sep 15, 2011·Cell Communication & Adhesion·Sabrina Roth, Riccardo Fodde
Nov 3, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Carl SimonssonJean-Pierre Benoit
Aug 20, 2005·Stem Cells·Timothy R Brazelton, Helen M Blau
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Sep 5, 2008·Current Protocols in Cytometry·I D Johnson
Jan 25, 2020·Scientific Reports·Catherine CardWitold W Kilarski

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