Cell migration and polarity on microfabricated gradients of extracellular matrix proteins

Langmuir : the ACS Journal of Surfaces and Colloids
Rico C GunawanDeborah E Leckband

Abstract

This paper explores the effects of the surface density and concentration profiles of extra cellular matrix proteins on the migration of rat intestinal IEC-6 cells. Microfluidic devices were used to create linear, immobilized gradients of laminin. This study investigated both the impact of the steepness and local concentrations on the directedness of cell migration. The bulk concentrations of proteins in the feed streams in the mixing device determined the gradient profile and the local concentration of laminin in the device. Two sets of gradients were used to explore cell migration directedness: (i) gradients with similar change in local concentration, i.e., the same gradient steepness, and (ii) different gradients with similar local concentrations. Cells migrated up the gradients, independent of the steepness of the gradients used in this study. At the same local laminin concentration, the migration rate was independent of the gradient steepness. However, cell directedness decreased significantly at high laminin densities.

References

Sep 1, 1992·The American Journal of Physiology·S A McCormackL R Johnson
Jul 1, 1991·Biophysical Journal·P A DiMillaD A Lauffenburger
Dec 1, 1986·The Journal of Cell Biology·F Grinnell
Apr 15, 1985·International Journal of Cancer. Journal International Du Cancer·J VaraniP Perone
Apr 1, 1984·The Journal of Cell Biology·J B McCarthy, L T Furcht
Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
Sep 1, 1996·The Journal of Cell Biology·A HuttenlocherA F Horwitz
Feb 1, 1997·In Vitro Cellular & Developmental Biology. Animal·B Wòjciak-StothardR A Brown
Jul 31, 1998·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C S Potten
Aug 19, 2000·Cellular and Molecular Life Sciences : CMLS·P Friedl, E B Bröcker
Sep 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Stephan K W DertingerGeorge M Whitesides
Jun 12, 2003·Experimental Cell Research·Thomas HelleBurkhard Schlosshauer
Mar 23, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Rico C GunawanPaul J A Kenis

❮ Previous
Next ❯

Citations

Dec 7, 2013·Molecules and Cells·Morgan Hamon, Jong Wook Hong
Mar 23, 2011·Annals of Biomedical Engineering·Ngan F Huang, Song Li
Jan 24, 2012·Analytical Chemistry·Yousef AwwadChang Lu
Aug 30, 2013·Analytical Chemistry·Mei ZhanHang Lu
Sep 7, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Rico C GunawanWilliam M Miller
Jul 9, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Jonathan SilvestreDeborah E Leckband
Oct 20, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Sudong KimNoo Li Jeon
Sep 23, 2008·Tissue Engineering. Part B, Reviews·Milind SinghMichael S Detamore
Jun 7, 2013·Interface Focus·Jindan WuChangyou Gao
Feb 7, 2014·Interface Focus·Beatrice ChelliFabio Biscarini
Apr 30, 2009·Annual Review of Biomedical Engineering·Deok-Ho KimYu Sun
Apr 5, 2014·ACS Applied Materials & Interfaces·Sudeshna RoyRabibrata Mukherjee
Mar 25, 2014·Colloids and Surfaces. B, Biointerfaces·Jindan WuChangyou Gao
Apr 5, 2011·Annual Review of Analytical Chemistry·Jason S Kuo, Daniel T Chiu
Jan 15, 2015·Biomicrofluidics·Miguel A AcostaMichael P Gamcsik
Jul 16, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Christine R TohRyan C Bailey
Dec 24, 2010·Biomaterials·Yaseen M ElkasabiPaul H Krebsbach
May 14, 2010·Colloids and Surfaces. B, Biointerfaces·Kaiyong CaiChong Chen
Jun 16, 2010·Journal of Bioscience and Bioengineering·Tomoaki OkuyamaJunji Fukuda
Dec 6, 2008·Bio Systems·L Leon ChenThomas S Deisboeck
Oct 23, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Annalisa TirellaArti Ahluwalia
Feb 11, 2009·Biotechnology and Bioengineering·Alireza S Sarvestani, Esmaiel Jabbari
Feb 11, 2009·Biotechnology Progress·Danny van NoortHanry Yu
Sep 3, 2013·Advanced Healthcare Materials·Bhushan P MahadikBrendan A C Harley
Dec 23, 2014·Advanced Functional Materials·Greg M HarrisEhsan Jabbarzadeh
Aug 25, 2009·Biotechnology and Bioengineering·Alireza S Sarvestani
Dec 21, 2007·Lab on a Chip·Thomas M Keenan, Albert Folch
Dec 20, 2011·Lab on a Chip·Kwang W OhEdward P Furlani
Aug 10, 2016·Tissue & Cell·You-Quan DingJian-Guo Qi
Aug 16, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Stephanie Hiltl, Alexander Böker
Aug 24, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Jordi ComellesElena Martínez
May 28, 2013·Cellular and Molecular Bioengineering·Xiyi ChenPaul J Campagnola
Jun 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Edmondo M BenettiLorenzo Moroni
Aug 7, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Alexandra KhlyustovaRong Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin, Myosin & Cell Movement

Contractile forces generated by the actin-myosin cytoskeleton are critical for morphogenesis, but the cellular and molecular mechanisms of contraction have been elusive for many cell shape changes and movements. Here is the latest research on the roles of actin and myosin in cell movement.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.