PMID: 8972713Nov 8, 1996Paper

Cell migration promoted by a potent GRGDS-containing thrombin-cleavage fragment of osteopontin

Biochimica Et Biophysica Acta
D R Senger, C A Perruzzi

Abstract

Osteopontin (OPN) is a secreted adhesive glycoprotein with a gly-arg-gly-asp-ser (GRGDS) cell binding domain. Several independent studies have suggested that OPN functions in tumor growth and metastasis, and one likely possibility is that OPN facilitates tumor invasion by promoting tumor cell migration. Consistent with this hypothesis, immobilized OPN promoted concentration-dependent tumor cell migration (i.e., haptotaxis) in modified Boyden chambers. In particular, cleavage of OPN by thrombin, which likely occurs in the tumor microenvironment, resulted in enhancement of OPNs haptotactic activity; and assays performed with purified preparations of the two individual OPN thrombin-cleavage fragments demonstrated that all detectable activity was associated with the GRGDS-containing fragment. In contrast to the activity of both OPN and its GRGDS-containing fragment in promoting haptotaxis, neither of these proteins in solution promoted chemotaxis, indicating that each must be immobilized to promote cell migration. In haptotaxis assays, antibody LM609 to integrin alpha v beta 3 blocked > 80% cell migration towards the GRGDS-containing OPN fragment, implicating alpha v beta 3 as its principal functional receptor. In comparison with e...Continue Reading

References

Dec 4, 1992·Annals of the New York Academy of Sciences·H F DvorakD R Senger
Jun 1, 1992·The Journal of Clinical Investigation·B Felding-HabermannD A Cheresh
Jun 28, 1991·Biochemical and Biophysical Research Communications·C W PrinceC L Krumdieck
May 1, 1991·Cancer Metastasis Reviews·R H KramerD M Ramos
Jun 1, 1991·Cancer Metastasis Reviews·I R HartJ F Marshall
Jan 1, 1989·Matrix : Collagen and Related Research·M J SomermanJ J Sauk
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A OldbergD Heinegård
Nov 15, 1994·The Biochemical Journal·E S SørensenT E Petersen

❮ Previous
Next ❯

Citations

Aug 20, 2009·Cancer Immunology, Immunotherapy : CII·Jianxin DaiYajun Guo
Dec 1, 2007·Cancer Metastasis Reviews·Philip Y Wai, Paul C Kuo
Aug 19, 2003·Tissue & Cell·K V R Reddy, Sachin S Mangale
Feb 5, 2002·Biochimica Et Biophysica Acta·G F Weber
Oct 18, 2003·Cytokine & Growth Factor Reviews·Anthony O'Regan
Jun 13, 2009·Biochemistry·Paul CurtinErdjan Salih
Jan 24, 2002·Breast Cancer Research and Treatment·A B TuckA F Chambers
Oct 7, 2005·Leukemia·V Cheriyath, M A Hussein
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·D PandaA B Mukherjee
Sep 14, 2010·The Journal of Biological Chemistry·Hai-Yan ZhouMelvin J Glimcher
Apr 17, 2008·The Journal of Biological Chemistry·Timothy Myles, Lawrence L K Leung
Aug 31, 2002·Tissue Engineering·Janine M OrbanJeffrey O Hollinger
Sep 2, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniel J SchneiderMichael R Blackburn
Aug 3, 2011·Cold Spring Harbor Perspectives in Biology·Donald R Senger, George E Davis
Feb 8, 2003·American Journal of Respiratory and Critical Care Medicine·Yoshinobu KoguchiAtsushi Saito
Jan 10, 2014·Journal of Diabetes and Metabolic Disorders·Subramaniam Sivakumar, Sivasitambaram Niranjali Devaraj
Oct 3, 2009·Journal of Cell Communication and Signaling·Susan Amanda LundMarta Scatena
Apr 12, 2001·International Journal of Experimental Pathology·A O'Regan, J S Berman
Oct 17, 2009·Journal of Cancer Research and Clinical Oncology·Jin-Cai WuLun-Xiu Qin
May 23, 2012·Journal of Cancer Research and Clinical Oncology·Miriam E UhlmannMartin R Berger
Jan 30, 2015·Cancers·Theodora Stivarou, Evangelia Patsavoudi
Feb 16, 2010·Biochemical and Biophysical Research Communications·Yoshiki YamaguchiKoichi Kato
Jun 2, 2009·Seminars in Cancer Biology·Chiara FrancavillaUgo Cavallaro
Oct 30, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kathleen DeiterenAnne Marie Lambeir
Sep 1, 2007·International Immunopharmacology·Nobuchika YamamotoToshimitsu Uede
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·Divya PankajakshanLissy K Krishnan
Oct 5, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yu-Hua XueLun-Xiu Qin
Sep 20, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nilana M T BarrosMarc D McKee
Oct 19, 2011·The Journal of Pathology·Pavlo G GritsenkoPeter Friedl
Nov 18, 2000·Experimental Cell Research·G Orend, R Chiquet-Ehrismann
Oct 27, 2004·The Journal of Surgical Research·Philip Y Wai, Paul C Kuo
Mar 27, 2012·Journal of Experimental & Clinical Cancer Research : CR·Farbod ShojaeiEugenia Kraynov
Jan 7, 2014·Differentiation; Research in Biological Diversity·Fuller W Bazer, Gregory A Johnson
Jan 5, 2002·Cell Communication & Adhesion·N SogaK A Hruska
Aug 30, 2001·Experimental Cell Research·N SogaK A Hruska
Feb 12, 2015·Journal of Animal Science and Biotechnology·Greg A JohnsonFuller W Bazer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.