Abstract
Chemotaxis or directed cell migration is mediated by signalling events initiated by binding of chemokines to their cognate receptors and the activation of a complex signalling cascade. The molecular signalling pathways involved in cell migration are important to understand cancer cell metastasis. Therefore, we investigated the molecular mechanisms of CXCL12 induced cell migration and the importance of different signalling cascades that become activated by CXCR4 in leukemic cells versus breast cancer cells. We identified Src kinase as being essential for cell migration in both cancer types, with strong involvement of the Raf/MEK/ERK1/2 pathway. We did not detect any involvement of Ras or JAK2/STAT3 in CXCL12 induced migration in Jurkat cells. Preventing PKC activation with inhibitors does not affect migration in Jurkat cells at all, unlike in the adherent breast cancer cell line MCF-7 cells. However, in both cell lines, knock down of PKCα prevents migration towards CXCL12, whereas the expression of PKCζ is less crucial for migration. PI3K activation is essential in both cell types, however LY294002 usage in MCF-7 cells does not block migration significantly. These results highlight the importance of verifying specific signalling...Continue Reading
References
Oct 31, 2002·The Journal of Biological Chemistry·Beatrix Pollok-KoppMartin Oppermann
Jul 6, 2004·Oncogene·Frank J M OpdamEd Roos
May 4, 2006·International Journal of Cancer. Journal International Du Cancer·Albert Zlotnik
Aug 19, 2007·Trends in Pharmacological Sciences·Stephen P Soltoff
Feb 13, 2008·Cell·Bijan BoldajipourErez Raz
Apr 26, 2008·Blood·Bernard LaganeFrançoise Bachelerie
Mar 6, 2009·Nature Reviews. Cancer·Kornelia Polyak, Robert A Weinberg
Jul 4, 2009·Cancer Cell·Xiang H-F ZhangJoan Massagué
May 21, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Beverly A Teicher, Simon P Fricker
Sep 22, 2010·Oncogene·J KimD Mochly-Rosen
Dec 3, 2010·PloS One·David A KnechtGabriel Fenteany
Jan 13, 2011·Experimental Cell Research·Dayanidhi RamanAnn Richmond
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Sep 19, 2012·Cell Biochemistry and Function·S KhabbaziA Mueller
Sep 17, 2013·Clinical Lymphoma, Myeloma & Leukemia·Sergej KonoplevMarina Konopleva
Nov 10, 2013·Cell Cycle·Antonella De LucaNicola Normanno
Jan 10, 2014·Molecular Biology of the Cell·Tammy SobolikAnn Richmond
Feb 22, 2014·Cell Death & Disease·L PatrussiC T Baldari
Mar 7, 2014·PloS One·Ishan RoyMichael B Dwinell
Jun 5, 2014·PloS One·Nathaniel L CogginsGary D Luker
Dec 4, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Patricia Dillenburg-PillaJ Silvio Gutkind
Jan 13, 2015·Cellular and Molecular Life Sciences : CMLS·Aparecida de Lourdes PerimMaria Angelica Ehara Watanabe
Citations
Jan 10, 2017·Scientific Reports·Mingwei LiQing Yu
Nov 9, 2018·Oncology Letters·Jixian LuoLan Wang
Mar 12, 2019·Chinese Medical Journal·Xiang-Long MengZhuo Li
Apr 2, 2019·MRS Communications·Annamarija RaicCornelia Lee-Thedieck
Jul 18, 2018·BMC Cancer·Cláudia S MarquesFernando Ferreira
Sep 4, 2019·Biochemical and Biophysical Research Communications·I HamshawA Mueller
Dec 1, 2019·Cellular Signalling·Khanh T P NguyenDidier Dréau
Dec 25, 2019·Seminars in Cancer Biology·Sara K DanielVenu G Pillarisetty
Sep 22, 2020·Annual Review of Pharmacology and Toxicology·Martine J SmitFederico Mayor
Aug 11, 2020·European Journal of Pharmacology·Enana Alassaf, Anja Mueller