Cell morphology, albumin secretion and glutathione S-Transferase expression in collagen gel sandwich and immobilization cultures of rat hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
S BekenV Rogiers

Abstract

In order to investigate whether collagen gel sandwich and immobilization cultures of rat hepatocytes are suitable in vitro models for long-term pharmaco-toxicological studies, the expression of the key phase II biotransformation enzyme, glutathione S-transferase (GST, EC 2.5.1.18), has been studied in the presence or absence of l-proline (60mug/ml) in the culture medium. Additionally, hepatocytes morphology was followed and albumin secretion into the medium measured. As judged by inverse phase light microscopy and transmission electron microscopy, cells cultured in both organotypical models remained viable and well differentiated for at least 14 days. Albumin secretion increased 2.5-fold after 7 days of culture, in comparison with the values found after 2 days, and remained thereafter relatively constant. When l-proline was added to the medium of sandwich and immobilization gel cultures, steady-state secretion levels of 7.1 and 5.1 mug albumin/hr, respectively, were already obtained after 4 days of culture. Total, Mu, Alpha and Pi class GST activities were determined using a general substrate and isoenzyme specific substrates, respectively. After 7 days of culture, total GST activities were decreased as compared with the values...Continue Reading

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Citations

May 4, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E L LeCluyseJ Johnson
Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T VanhaeckeV Rogiers
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S BekenV Rogiers
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K De SmetV Rogiers
Nov 29, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·N TreijtelA P Freidig
Mar 28, 2008·Journal of Cellular and Molecular Medicine·A Jeremic
Jul 20, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Nicoline TreijtelJan C H van Eijkeren
Jun 2, 2001·Alternatives to Laboratory Animals : ATLA·T VanhaeckeV Rogiers
Jul 1, 1999·Alternatives to Laboratory Animals : ATLA·S CoeckeC Westmoreland
Jun 14, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Nicoline TreijtelJan C H van Eijkeren

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