Cell motility regulation on a stepped micro pillar array device (SMPAD) with a discrete stiffness gradient

Soft Matter
Sujin LeeJunghoon Lee

Abstract

Our tissues consist of individual cells that respond to the elasticity of their environment, which varies between and within tissues. To better understand mechanically driven cell migration, it is necessary to manipulate the stiffness gradient across a substrate. Here, we have demonstrated a new variant of the microfabricated polymeric pillar array platform that can decouple the stiffness gradient from the ECM protein area. This goal is achieved via a "stepped" micro pillar array device (SMPAD) in which the contact area with the cell was kept constant while the diameter of the pillar bodies was altered to attain the proper mechanical stiffness. Using double-step SU-8 mold fabrication, the diameter of the top of every pillar was kept uniform, whereas that of the bottom was changed, to achieve the desired substrate rigidity. Fibronectin was immobilized on the pillar tops, providing a focal adhesion site for cells. C2C12, HeLa and NIH3T3 cells were cultured on the SMPAD, and the motion of the cells was observed by time-lapse microscopy. Using this simple platform, which produces a purely physical stimulus, we observed that various types of cell behavior are affected by the mechanical stimulus of the environment. We also demonstrat...Continue Reading

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Citations

Mar 17, 2017·Molecular Biology of the Cell·Lauren D'AngeloKenneth A Myers
Jun 20, 2017·Nature Cell Biology·Pere Roca-CusachsXavier Trepat
Jul 7, 2017·The European Respiratory Journal·Gerald BurgstallerOliver Eickelberg
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Feb 3, 2019·Biophysical Journal·Brian J DuChezKenneth M Yamada
Jun 13, 2020·ACS Chemical Biology·Ashley K Nguyen, Kristopher A Kilian

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