Cell movement is guided by the rigidity of the substrate

Biophysical Journal
C M LoY L Wang

Abstract

Directional cell locomotion is critical in many physiological processes, including morphogenesis, the immune response, and wound healing. It is well known that in these processes cell movements can be guided by gradients of various chemical signals. In this study, we demonstrate that cell movement can also be guided by purely physical interactions at the cell-substrate interface. We cultured National Institutes of Health 3T3 fibroblasts on flexible polyacrylamide sheets coated with type I collagen. A transition in rigidity was introduced in the central region of the sheet by a discontinuity in the concentration of the bis-acrylamide cross-linker. Cells approaching the transition region from the soft side could easily migrate across the boundary, with a concurrent increase in spreading area and traction forces. In contrast, cells migrating from the stiff side turned around or retracted as they reached the boundary. We call this apparent preference for a stiff substrate "durotaxis." In addition to substrate rigidity, we discovered that cell movement could also be guided by manipulating the flexible substrate to produce mechanical strains in the front or rear of a polarized cell. We conclude that changes in tissue rigidity and str...Continue Reading

References

Jul 13, 1989·Nature·P LamoureuxS R Heidemann
Mar 15, 1973·Experimental Cell Research·A Harris
Jan 21, 1967·Nature·S B Carter
Jan 1, 1984·The Journal of Cell Biology·C A Erickson, R Nuccitelli
Mar 1, 1995·Experimental Cell Research·N L Halliday, J J Tomasek
Feb 1, 1993·The Journal of Cell Biology·R L Juliano, S Haskill
Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
Sep 1, 1996·The Journal of Cell Biology·A HuttenlocherA F Horwitz
May 29, 1997·Nature·J Saranak, K W Foster
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J Pelham, Y l Wang
Jun 5, 1998·Biomaterials·A Curtis, C Wilkinson
Aug 8, 1998·Trends in Cell Biology·M P SheetzC G Galbraith
Oct 28, 1998·Physiological Reviews·E J Pettit, F S Fay
Jan 16, 1999·Current Biology : CB·A B VerkhovskyG G Borisy
Mar 30, 1999·Biophysical Journal·M Dembo, Y L Wang
Apr 10, 1999·Molecular Biology of the Cell·R J Pelham, Y l Wang
Oct 6, 1999·Current Opinion in Cell Biology·J E Schwarzbauer, J L Sechler

❮ Previous
Next ❯

Citations

Dec 2, 2004·Cell Motility and the Cytoskeleton·Tony YeungPaul A Janmey
Dec 13, 2006·European Biophysics Journal : EBJ·L VonnaE Sackmann
Jun 3, 2009·European Biophysics Journal : EBJ·Juliane Loichen, Uwe Hartmann
Jul 7, 2009·European Biophysics Journal : EBJ·Cécile CouzonClaude Verdier
Apr 9, 2008·Journal of Mathematical Biology·D AmbrosiC Verdier
May 20, 2008·Journal of Mathematical Biology·Esa Kuusela, Wolfgang Alt
Dec 17, 2009·Journal of Mathematical Biology·Andreas Groh, Alfred K Louis
Aug 14, 2012·Archives of Dermatological Research·Chenyu HuangRei Ogawa
Dec 7, 2007·Pflügers Archiv : European journal of physiology·Thomas LudwigUlrich S Schwarz
Mar 26, 2011·Pflügers Archiv : European journal of physiology·J Yasha Kresh, Anant Chopra
Apr 25, 2012·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Irza Sukmana
Jan 5, 2007·Biomechanics and Modeling in Mechanobiology·James H-C Wang, Jeen-Shang Lin
Jul 10, 2007·Biomechanics and Modeling in Mechanobiology·G P RaeberJ A Hubbell
Feb 20, 2010·Biomechanics and Modeling in Mechanobiology·Jenna L BalestriniKristen L Billiar
Aug 20, 2010·Biomechanics and Modeling in Mechanobiology·Yasuhiro Inoue, Taiji Adachi
Dec 31, 2010·Biomechanics and Modeling in Mechanobiology·M R NelsonS L Waters
Mar 29, 2011·Biomechanics and Modeling in Mechanobiology·F J Vermolen, A Gefen
Jan 2, 2013·Biomechanics and Modeling in Mechanobiology·R Rey, J M García-Aznar
Mar 10, 2006·Annals of Biomedical Engineering·Nathan J SniadeckiChristopher S Chen
Dec 28, 2006·Annals of Biomedical Engineering·Charles Stevenson WallaceGeorge A Truskey
Jan 22, 2008·Annals of Biomedical Engineering·Sheng-Yang LeeHaw-Ming Huang
Oct 10, 2009·Annals of Biomedical Engineering·Stephanie Nemir, Jennifer L West
Apr 23, 2010·Annals of Biomedical Engineering·M D C Lopez-GarciaW C Crone
Jan 22, 2011·Annals of Biomedical Engineering·Adrian C Shieh
Mar 23, 2011·Annals of Biomedical Engineering·Ngan F Huang, Song Li
Dec 14, 2011·Annals of Biomedical Engineering·Michelle L PreviteraNoshir A Langrana
May 23, 2007·Biomedical Microdevices·Abel L ThangawngMatthew R Glucksberg
Apr 20, 2005·Journal of Mammary Gland Biology and Neoplasia·Matthew J Paszek, Valerie M Weaver
Jan 8, 2011·Pharmaceutical Research·Hillary Holback, Yoon Yeo
Jul 30, 2009·Medical & Biological Engineering & Computing·Shu-Li LinHaw-Ming Huang
May 18, 2013·Bulletin of Mathematical Biology·Mark S Thompson
Jul 27, 2007·Cell Biochemistry and Biophysics·Shelly R PeytonAndrew J Putnam
Sep 18, 2007·Cell Biochemistry and Biophysics·Brendan FlahertyP E McHugh
Mar 1, 2012·Archives of Pharmacal Research·Ji Won ShinJung-Woog Shin
Jan 7, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Daniel L WorthleyTimothy C Wang
Jul 23, 2013·Biomaterials·Soraya Rasi GhaemiNicolas H Voelcker
Sep 24, 2013·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Chenyu HuangRei Ogawa
Dec 13, 2003·Bulletin of Mathematical Biology·Maria E Gracheva, Hans G Othmer
Nov 21, 2013·European Journal of Cell Biology·Guillaume Romet-Lemonne, Antoine Jégou
Jun 14, 2005·Medical Engineering & Physics·Ulrich S Schwarz, Ilka B Bischofs
Oct 12, 2013·Experimental Cell Research·Seiichiro IshiharaHisashi Haga

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Actin, Myosin & Cell Movement

Contractile forces generated by the actin-myosin cytoskeleton are critical for morphogenesis, but the cellular and molecular mechanisms of contraction have been elusive for many cell shape changes and movements. Here is the latest research on the roles of actin and myosin in cell movement.

© 2021 Meta ULC. All rights reserved