Cell Profiling Based on Sugar-Chain-Cell Binding Interaction and Its Application to Typing and Quality Verification of Cells

Chembiochem : a European Journal of Chemical Biology
Hiroyuki ShinchiYasuo Suda

Abstract

Developing methods to determine cell type and cell state has been a significant challenge in the field of cancer diagnosis as well as in typing and quality verification for cultured cells. Herein, we report a cell profiling method based on binding interactions between cell-surface sugar-chain-binding proteins and sugar-chain-immobilized fluorescent nanoparticles (SFNPs), together with a method for cell typing and cell quality verification. Binding profiles of cells against sugar chains were analyzed by performing flow cytometry analysis with SFNPs. Discrimination analysis based on binding profiles could classify cell type and evaluate the quality of cultured cells. By applying our method to differentiated cells originating from conventional cell lines and also to mouse embryotic stem cells, we could detect the cells before and after differentiation. Our method can be utilized not only for the biofunctional analysis of cells but also for diagnosis of cancer cells and quality verification of cultured cells.

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S J CollinsR C Gallo
Feb 1, 1993·The Journal of Clinical Investigation·M P Bevilacqua, R M Nelson
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A NagyJ C Roder
Apr 19, 1996·The Journal of Biological Chemistry·K Panneerselvam, H H Freeze
May 30, 2001·Trends in Immunology·P R Crocker, A Varki
Mar 21, 2002·The Lancet Oncology·P R SrinivasS Srivastava
Mar 26, 2003·Glycobiology·Daniel J Becker, John B Lowe
Mar 12, 2004·Biochemical Pharmacology·Angelica ButuraIna Schuppe-Koistinen
Dec 13, 2006·Bulletin of Experimental Biology and Medicine·S N GornostaevaD V Gol'dstein
Dec 21, 2006·Differentiation; Research in Biological Diversity·Sarah TonackAnne Navarrete Santos
Aug 19, 2007·Glycobiology·Hiroaki TatenoJun Hirabayashi
Apr 12, 2008·Nature Reviews. Cancer·Klaus PantelBurkhard Brandt
Jul 29, 2008·Current Genomics·Feng-Qi Zhao, Aileen F Keating
Apr 3, 2009·Biochimica Et Biophysica Acta·Hudson H Freeze
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Avinash BajajVincent M Rotello
Mar 6, 2010·Journal of the American Chemical Society·Kheireddine El-BoubbouXuefei Huang
Mar 25, 2010·Glycobiology·Hirohiko IseToshihiro Akaike
Aug 26, 2010·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Kurt E J DittmarWerner Lindenmaier
Aug 31, 2010·Current Opinion in Chemical Biology·Oscar R MirandaVincent M Rotello
Mar 11, 2011·Nature Reviews. Cancer·Uri Ben-David, Nissim Benvenisty
Apr 30, 2011·Physiological Reviews·Ernest M WrightBruce A Hirayama
Jun 4, 2011·Cellular and Molecular Life Sciences : CMLS·Arun Satelli, Shulin Li
Aug 22, 2012·Chemistry, an Asian Journal·Hiroyuki ShinchiYasuo Suda
Feb 28, 2013·Chemical Society Reviews·Jun HirabayashiHiroaki Tateno
Mar 20, 2013·Molecular Aspects of Medicine·Mike Mueckler, Bernard Thorens
Mar 31, 2015·Biochimica Et Biophysica Acta·Victor L ThijssenArjan W Griffioen
May 6, 2015·Journal of the American Chemical Society·Sungjin ParkInjae Shin
Sep 26, 2015·ACS Central Science·Subinoy RanaVincent M Rotello
May 24, 2017·Journal of the American Chemical Society·Ngoc D B LeVincent M Rotello
Jan 14, 2018·European Journal of Pharmacology·Yui YamazakiShogo Tokuyama
Jul 22, 2018·Nature Communications·Joseph KononchikPeter Sun

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.