Cell proliferation and migration explain pore bridging dynamics in 3D printed scaffolds of different pore size

Acta Biomaterialia
Pascal R BuenzliMatthew J Simpson

Abstract

Tissue growth in bioscaffolds is influenced significantly by pore geometry, but how this geometric dependence emerges from dynamic cellular processes such as cell proliferation and cell migration remains poorly understood. Here we investigate the influence of pore size on the time required to bridge pores in thin 3D-printed scaffolds. Experimentally, new tissue infills the pores continually from their perimeter under strong curvature control, which leads the tissue front to round off with time. Despite the varied shapes assumed by the tissue during this evolution, we find that time to bridge a pore simply increases linearly with the overall pore size. To disentangle the biological influence of cell behaviour and the mechanistic influence of geometry in this experimental observation, we propose a simple reaction-diffusion model of tissue growth based on Porous-Fisher invasion of cells into the pores. First, this model provides a good qualitative representation of the evolution of the tissue; new tissue in the model grows at an effective rate that depends on the local curvature of the tissue substrate. Second, the model suggests that a linear dependence of bridging time with pore size arises due to geometric reasons alone, not to...Continue Reading

Citations

Sep 18, 2020·Advanced Healthcare Materials·Juliane C Kade, Paul D Dalton
Feb 22, 2021·Bulletin of Mathematical Biology·Maud El-HachemMatthew J Simpson
Mar 21, 2021·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·William HarleyCarmine Gentile
May 6, 2021·Macromolecular Bioscience·Andrei HrynevichPaul D Dalton
Jun 5, 2021·International Journal of Biological Macromolecules·Changjun LiuHongjun Yang
Aug 7, 2021·Journal of Theoretical Biology·Alexander P BrowningMatthew J Simpson

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