PMID: 2501790Jul 1, 1989Paper

Cell proliferation and thymocyte subset reconstitution in sublethally irradiated mice: compared kinetics of endogenous and intrathymically transferred progenitors

Proceedings of the National Academy of Sciences of the United States of America
C Penit, S Ezine

Abstract

After sublethal (6 Gy) whole-body irradiation, the C57BL/Ba (Thy-1.1) murine thymus regenerated in two waves, on days 3-10 and 25-32, separated by a severe relapse. The second phase of depletion-reconstitution reproduced the first one, in a less synchronous manner. The depletion affected all cell subsets, but CD4+ CD8- cells decreased later than immature cells. Cell proliferation, measured by BrdUrd incorporation, started on day 3 after irradiation and concerned CD4- CD8-, CD4- CD8+, and CD4+ CD8+ cells, sequentially. CD4+ CD8- cells never represented a significant percentage of cycling cells. When irradiation was immediately followed by an intrathymic injection of 10(5) C57BL/Ka (Thy-1.2) bone marrow cells, the relapse in thymus reconstitution was no longer observed. Detected with anti-Thy-1.2 antibodies, donor cells started cycling on day 14 and showed only one wave of proliferation. In these chimeras, recipient thymocytes behave exactly like thymocytes of solely irradiated mice. Intrathymically transferred CD4- CD8- thymocytes (10(5] showed the same proliferation kinetics as endogenous cells, with a peak in number on day 10 but completely disappeared from the thymus on days 14-21. These data reflect maturational differences ...Continue Reading

References

May 1, 1976·The Journal of Experimental Medicine·J L Kadish, R S Basch
Jul 1, 1975·The Journal of Experimental Medicine·N M Le Douarin, F V Jotereau
Jan 1, 1979·Immunological Reviews·J A Ledbetter, L A Herzenberg
Sep 1, 1985·The Journal of Experimental Medicine·B J FowlkesT M Chused
May 1, 1988·The Journal of Experimental Medicine·G J SpangrudeI L Weissman
Sep 24, 1987·Nature·I N CrispeR P Shimonkevitz
Jun 1, 1988·European Journal of Immunology·Y KatsuraS Nishikawa
Nov 1, 1987·The Journal of Experimental Medicine·D J Paterson, A F Williams
Nov 1, 1985·Developmental Biology·E Rothenberg, J P Lugo
Oct 1, 1967·The Journal of Experimental Medicine·M A Moore, J J Owen
Jan 1, 1982·Immunological Reviews·J A Ledbetter, W E Seaman

❮ Previous
Next ❯

Citations

May 1, 1994·Developmental and Comparative Immunology·G G Fredrickson, R S Basch
Dec 29, 1998·Immunology Today·C Tanchot, B Rocha
May 4, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C TanchotB Rocha
Apr 1, 2005·Radiation Research·Yasuhiro AdachiTetsuo Fukumoto
Jul 8, 2000·Archives of Histology and Cytology·Y ArudchelvanF Shinozaki
Oct 7, 1997·The Journal of Experimental Medicine·C Tanchot, B Rocha
Apr 30, 2017·EMBO Molecular Medicine·Noella LopesMagali Irla
Jul 29, 1999·The Journal of Veterinary Medical Science·S OkaY Takamori
Mar 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stuart P BerzinsDale I Godfrey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.