Cell purification: a new challenge for biobanks.

Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology
Maria AlmeidaAlberto Orfao

Abstract

Performing '-omics' analyses on heterogeneous biological tissue samples, such as blood or bone marrow, can lead to biased or even erroneous results, particularly when the targeted cells and/or molecules are present at relatively low percentages/amounts. In such cases, whole sample analysis will most probably dilute and mask the features of the cell and/or molecules of interest, and this will negatively impact the results and their interpretation. Therefore, frequently it is critically important to have well-characterized and high-quality purified cell populations for the reliable detection of subtle variations in their specific features, such as gene expression profile, protein expression pattern and metabolic status. Biobanks are technological platforms which aim to provide researchers access to a large number of high-quality biological samples and their associated data, particularly to support high-quality scientific and clinical research projects, and such projects will benefit enormously by having access to high-quality purified cell populations or their biological components (e.g. DNA, RNA, proteins). Therefore, a clear opportunity exists for preparative cell sorting techniques in biobanks. Although multiple different cell...Continue Reading

Citations

Aug 20, 2017·Cell and Tissue Banking·Andreas Nicodemou, Lubos Danisovic
Jun 10, 2017·Clinical and Translational Medicine·Raquel Cuevas-Diaz DuranJia Qian Wu
Dec 15, 2020·Lab on a Chip·Yu-Chun KungPei-Yu Chiou
May 7, 2021·Stem Cells International·Dina RadyKarim M Fawzy El-Sayed

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