PMID: 8593588Oct 30, 1995Paper

Cell selective induction and transcriptional activation of immediate early genes by hypoxia

Brain Research
Nanduri R PrabhakarN S Cherniack

Abstract

c-fos and jun belong to the immediate early response genes (IERG) that initiate phenotypic changes in response to a variety of extracellular stimuli. In the present study, we examined whether hypoxia induces IERG expression in isolated cells. Experiments were performed on pheochromocytoma-12 (PC-12), hepatoblastoma (Hep3B), neuroblastoma and fibroblast cells that were exposed either to normoxia (21% O2) or to hypoxia (5% O2) for one hour. mRNAs for c-fos, c-jun, junB, junD were analyzed by northern blot assay. Increases in IERG mRNAs were seen in PC-12, Hep3B, and fibroblasts but not in neuroblastoma cells. Significant induction of c-fos mRNA was seen with hypoxic exposure as short as 15 min and the effects persisted at 10 h of low pO2 exposure. Hypoxia stimulated transcription from a 356 bp fragment of the c-fos promoter linked to a choloramphenicol acetyl transferase reporter in PC-12 but not in neuroblastoma cells. Fetal bovine serum, however, activated c-fos promoter both in PC-12 and neuroblastoma cells. These results demonstrate cell type selective mechanisms for c-fos promoter activation that require nucleic acid sequences with in the first 356 bp of the c-fos promoter. These observations suggest that increased IERG tran...Continue Reading

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Citations

Dec 5, 2002·Physiological Genomics·Larry A SonnaCraig M Lilly
Aug 7, 1999·The Journal of Biological Chemistry·P W ConradD Beitner-Johnson
Oct 18, 2002·Microscopy Research and Technique·Waltke R PauldingMaria F Czyzyk-Krzeska
Jan 13, 2004·Journal of Applied Physiology·Karen A Seta, David E Millhorn
May 13, 2015·Developmental Neurobiology·Jeremy P LandryMaria Pompeiano
Jul 12, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Imre Hunyor, Kristina M Cook
Apr 27, 2004·The Journal of Physiology·Guoxiang YuanNanduri R Prabhakar
Aug 22, 2007·Molecular and Cellular Biology·Sandra BlancoPedro A Lazo
May 8, 2000·Journal of Applied Physiology·D R PremkumarN R Prabhakar
Mar 2, 1999·Microvascular Research·P Dore-DuffyJ C LaManna
Mar 21, 2001·Cellular Signalling·D Beitner-JohnsonD E Millhorn
Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M Hermes-LimaK B Storey
Sep 5, 1998·Brain Research. Molecular Brain Research·R R MishraN R Prabhakar
Jun 14, 2005·Respiratory Physiology & Neurobiology·Narong Simakajornboon, Teeradej Kuptanon
Feb 26, 2004·The American Journal of Pathology·Gen-ichi KusakaiHiroyasu Esumi

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