Cell Shape and Antibiotic Resistance Are Maintained by the Activity of Multiple FtsW and RodA Enzymes in Listeria monocytogenes

MBio
Jeanine RismondoAngelika Gründling

Abstract

Rod-shaped bacteria have two modes of peptidoglycan synthesis: lateral synthesis and synthesis at the cell division site. These two processes are controlled by two macromolecular protein complexes, the elongasome and divisome. Recently, it has been shown that the Bacillus subtilis RodA protein, which forms part of the elongasome, has peptidoglycan glycosyltransferase activity. The cell division-specific RodA homolog FtsW fulfils a similar role at the divisome. The human pathogen Listeria monocytogenes carries genes that encode up to six FtsW/RodA homologs; however, their functions have not yet been investigated. Analysis of deletion and depletion strains led to the identification of the essential cell division-specific FtsW protein, FtsW1. Interestingly, L. monocytogenes carries a gene that encodes a second FtsW protein, FtsW2, which can compensate for the lack of FtsW1, when expressed from an inducible promoter. L. monocytogenes also possesses three RodA homologs, RodA1, RodA2, and RodA3, and their combined absence is lethal. Cells of a rodA1 rodA3 double mutant are shorter and have increased antibiotic and lysozyme sensitivity, probably due to a weakened cell wall. Results from promoter activity assays revealed that expressio...Continue Reading

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Citations

Mar 19, 2020·Molecular Microbiology·Francisco García-Del Portillo
Jan 11, 2020·Scientific Reports·Elena Rivas-MarinDamien P Devos
Nov 25, 2020·Journal of Bacteriology·Jeanine RismondoAngelika Gründling
Nov 22, 2019·ACS Chemical Biology·Liubov Yakovlieva, Marthe T C Walvoort
Feb 16, 2021·Current Opinion in Microbiology·Adrien Ducret, Christophe Grangeasse
Oct 15, 2020·MBio·Elizabeth A Mueller, Petra Anne Levin
Feb 25, 2020·Journal of Proteome Research·Adithi R VaradarajanBernd Wollscheid

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Methods Mentioned

BETA
fluorescence microscopy
two-hybrid
environmental stresses
PCR
Fluorescence

Software Mentioned

Agilent Technology ChemStation
BLAST
Zen

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