Cell shape and the presentation of adhesion ligands guide smooth muscle myogenesis

Journal of Biomedical Materials Research. Part a
Douglas ZhangKristopher A Kilian

Abstract

The reliable generation of smooth muscle cells is important for a number of tissue engineering applications. Human mesenchymal stem cells (MSCs) are a promising progenitor of smooth muscle, with high expression of smooth muscle markers observed in a fraction of isolated cells, which can be increased by introduction of soluble supplements that direct differentiation. Here we demonstrate a new micropatterning technique, where peptides of different ligand affinity can be microcontact printed onto an inert background, to explore MSC differentiation to smooth muscle through controlled biochemical and biophysical cues alone. Using copper-catalyzed alkyne-azide cycloaddition (CuAAC), we patterned our surfaces with RGD peptide ligands-both a linear peptide with low integrin affinity and a cyclic version with high integrin affinity-for the culture of MSCs in shapes with various aspect ratios. At low aspect ratio, ligand affinity is a prime determinant for smooth muscle differentiation, while at high aspect ratio, ligand affinity has less of an effect. Pathway analysis reveals a role for focal adhesion turnover, Rac1, RhoA/ROCK, and calpain during smooth muscle differentiation of MSCs in response to cell shape and the affinity of the cel...Continue Reading

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Citations

Feb 13, 2018·Advanced Materials·Megan E CookeLiam M Grover
Dec 22, 2017·Journal of Tissue Engineering and Regenerative Medicine·Frank H KungBonnie L Firestein
Dec 9, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·K M PawelecR E Cameron
Mar 2, 2017·Journal of Biomedical Materials Research. Part a·Kendell M PawelecSebastiaan G J M Kluijtmans
Sep 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yingjun YangGuoping Chen
Apr 10, 2021·International Journal of Biological Macromolecules·Guanghui Ren, Guorong Li
Jun 3, 2017·ACS Central Science·Douglas ZhangKristopher A Kilian

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