Cell shape characterization and classification with discrete Fourier transforms and self-organizing maps

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Fabian L KriegelZoltan Cseresnyes

Abstract

Cells in their natural environment often exhibit complex kinetic behavior and radical adjustments of their shapes. This enables them to accommodate to short- and long-term changes in their surroundings under physiological and pathological conditions. Intravital multi-photon microscopy is a powerful tool to record this complex behavior. Traditionally, cell behavior is characterized by tracking the cells' movements, which yields numerous parameters describing the spatiotemporal characteristics of cells. Cells can be classified according to their tracking behavior using all or a subset of these kinetic parameters. This categorization can be supported by the a priori knowledge of experts. While such an approach provides an excellent starting point for analyzing complex intravital imaging data, faster methods are required for automated and unbiased characterization. In addition to their kinetic behavior, the 3D shape of these cells also provide essential clues about the cells' status and functionality. New approaches that include the study of cell shapes as well may also allow the discovery of correlations amongst the track- and shape-describing parameters. In the current study, we examine the applicability of a set of Fourier compo...Continue Reading

References

Jan 30, 2008·Medical Image Analysis·Frithjof Kruggel
Jan 1, 1995·IEEE Transactions on Neural Networks·Y K Kim, J B Ra
Feb 16, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·C Xu, J L Prince
Jan 22, 2009·Evolution; International Journal of Organic Evolution·Li ShenMark A McPeek
May 25, 2010·BMC Bioinformatics·Benjamin SchmidMartin Heisenberg
Sep 10, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Raluca A Niesner, Anja E Hauser
May 1, 2012·Translational Neuroscience·Emily L WilliamsManuel F Casanova
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Sep 21, 2012·Bioarchitecture·Andrius MasedunskasRoberto Weigert
May 29, 2013·Advanced Healthcare Materials·Haiyang YuWinston Shim
Sep 12, 2015·Molecular Biology of the Cell·Gregory R JohnsonRobert F Murphy

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Citations

May 8, 2018·International Journal of Molecular Sciences·Randall L LindquistAnja E Hauser
Apr 10, 2020·Scientific Reports·Anna MedyukhinaMarc Thilo Figge
Apr 21, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Attila Tárnok
Jun 2, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ivan BelyaevMarc Thilo Figge

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