Cell sheet composed of adipose-derived stem cells demonstrates enhanced skin wound healing with reduced scar formation

Acta Biomaterialia
Jiashing YuNai-Chen Cheng

Abstract

Scar formation remains a major clinical concern following tissue injuries such as skin wounds. Adipose-derived stem cell (ASC) sheets can be fabricated quickly through stimulation with l-ascorbate 2-phosphate and have valuable applications in tissue regeneration and wound healing. However, the antifibrotic capability of ASCs in cell sheet format has not been sufficiently investigated. We employed a murine model of healing-impaired cutaneous wounds and observed faster wound healing with ASC sheet treatment. Significantly more engrafted ASCs were observed in the wound tissue treated with ASC sheets at 14 days after wounding compared with dissociated cells. Moreover, no ASCs were found at day 28, which indicated a minimal risk of long-term side effects. The neoskin formed in the presence of ASC sheets exhibited a thickness comparable to normal skin and possessed a highly organized collagen structure. ASC sheets also suppressed macrophage infiltration and modulated TNF-α and TGF-β1 expression in vivo. Examination of fibroblasts cultured in ASC-conditioned medium indicated an anti-scarring effect of the ASC sheets evidenced by the downregulation of TGF-β1 and α-SMA in fibroblasts, which was likely mediated through the increased secr...Continue Reading

Citations

May 20, 2020·Journal of Plastic Surgery and Hand Surgery·Maria T HuayllaniAntonio J Forte
Jul 2, 2019·Experimental and Therapeutic Medicine·Shumei QiHui Chen
Jul 10, 2019·Npj Regenerative Medicine·Kihoon NamOlga J Baker
Jul 12, 2020·Journal of Tissue Engineering and Regenerative Medicine·Sonja MertschStefan Schrader
Mar 18, 2020·Frontiers in Bioengineering and Biotechnology·Qixin Han, Yanzhi Du
Nov 5, 2020·Nanomaterials·Alexandra Elena StoicaBogdan Stefan Vasile
Aug 6, 2019·Biotechnology Advances·Hao XingThemis R Kyriakides
Nov 29, 2020·Tissue & Cell·Hamed NosratiMohammad Khodaei
Dec 18, 2021·Tissue Engineering. Part B, Reviews·Adam Singer
Aug 20, 2021·Tissue Engineering. Part B, Reviews·Bihang ZhangKotaro Yoshimura

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