Cell size sets the diameter of the budding yeast contractile ring.

Nature Communications
I V KukhtevichK M Schmoller

Abstract

The formation and maintenance of subcellular structures and organelles with a well-defined size is a key requirement for cell function, yet our understanding of the underlying size control mechanisms is limited. While budding yeast cell polarization and subsequent assembly of a septin ring at the site of bud formation has been successfully used as a model for biological self-assembly processes, the mechanisms that set the size of the septin ring at the bud neck are unknown. Here, we use live-cell imaging and genetic manipulation of cell volume to show that the septin ring diameter increases with cell volume. This cell-volume-dependence largely accounts for modulations of ring size due to changes in ploidy and genetic manipulation of cell polarization. Our findings suggest that the ring diameter is set through the dynamic interplay of septin recruitment and Cdc42 polarization, establishing it as a model for size homeostasis of self-assembling organelles.

References

Jan 25, 2002·The Journal of Cell Biology·Amy S GladfelterDaniel J Lew
Jun 29, 2007·Molecular Biology of the Cell·Paul JorgensenBruce Futcher
Nov 14, 2007·The Journal of Cell Biology·Frank R Neumann, Paul Nurse
Apr 3, 2008·FEBS Letters·Andrew B Goryachev, Alexandra V Pokhilko
Jul 29, 2008·Nature·Zhanna ShcheprovaYves Barral
Aug 18, 2009·Current Biology : CB·Yuki Hara, Akatsuki Kimura
Dec 22, 2010·The Journal of Cell Biology·Xiaodong FangErfei Bi
Nov 30, 2011·The Journal of Cell Biology·Meredith E K CalvertMohan K Balasubramanian
May 12, 2012·Current Biology : CB·Nathan W Goehring, Anthony A Hyman
Jun 16, 2012·Genetics·Erfei Bi, Hay-Oak Park
Nov 10, 2012·Science·Susanne M RafelskiWallace F Marshall
Mar 23, 2013·PloS One·Andreas DoncicJan M Skotheim
May 9, 2013·Nature Communications·Tina FreisingerRoland Wedlich-Söldner
Aug 3, 2013·Developmental Cell·Satoshi OkadaAndrew B Goryachev
May 9, 2014·Biophysical Journal·Yee-Hung Mark Chan, Wallace F Marshall
Nov 25, 2014·Cell·Franz MeitingerGislene Pereira
Sep 22, 2015·Nature·Kurt M SchmollerJan M Skotheim
Oct 7, 2015·Current Biology : CB·Daria BonazziNicolas Minc
May 21, 2016·Molecular Cell·Jennifer C EwaldJan M Skotheim
Apr 19, 2017·ELife·Youlian GoulevGilles Charvin
Dec 13, 2017·Current Opinion in Cell Biology·Kurt M Schmoller
Jun 22, 2018·Molecular Biology of the Cell·Helen LaiDaniel J Lew
Jul 7, 2018·Cytoskeleton·Joseph MarquardtErfei Bi
Nov 22, 2018·The Journal of Cell Biology·Kyle D MoranDaniel J Lew
Jan 20, 2019·The Journal of Cell Biology·Kevin S CannonAmy S Gladfelter

❮ Previous
Next ❯

Citations

Nov 15, 2020·Current Opinion in Cell Biology·Benjamin L Woods, Amy S Gladfelter
Jul 11, 2021·Nature Communications·Kora-Lee ClaudeKurt M Schmoller

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPase
nucleotide-exchange
fluorescence microscopy
confocal microscopy

Software Mentioned

scikit
Elements
Phylocell
Fiji
NIS
image
Zen
Matlab

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.