Cell-Sized confinement in microspheres accelerates the reaction of gene expression.

Scientific Reports
Ayako KatoK Yoshikawa

Abstract

Cell-sized water-in-oil droplet covered by a lipid layer was used to understand how lipid membranes affect biochemical systems in living cells. Here, we report a remarkable acceleration of gene expression in a cell-sized water-in-oil droplet entrapping a cell-free translation system to synthesize GFP (green fluorescent protein). The production rate of GFP (V(GFP)) in each droplet remained almost constant at least for on the order of a day, which implies 0(th)-order reaction kinetics. Interestingly, V(GFP) was inversely proportional to radius of droplets (R) when R is under 50 μm, and V(GFP) in droplets with R ∼ 10 μm was more than 10 times higher than that in the bulk. The acceleration rates of GFP production in cell-sized droplets strongly depended on the lipid types. These results demonstrate that the membrane surface has the significant effect to facilitate protein production, especially when the scale of confinement is on the order of cell-size.

References

Feb 23, 1995·Nature·R HeimR Y Tsien
Sep 5, 1994·FEBS Letters·S Inouye, F I Tsuji
Jul 30, 1999·Biochemical and Biophysical Research Communications·T OberholzerP L Luisi
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·H SprongG van Meer
Aug 2, 2001·Nature Biotechnology·Y ShimizuT Ueda
Nov 13, 2003·Chembiochem : a European Journal of Chemical Biology·Shin-ichiro M NomuraKenichi Yoshikawa
Aug 10, 2004·Chembiochem : a European Journal of Chemical Biology·Adriana V Pietrini, Pier Luigi Luisi
Oct 23, 2004·FEBS Letters·Keitaro IshikawaTetsuya Yomo
Dec 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Vincent Noireaux, Albert Libchaber
Oct 20, 2005·Journal of Bioscience and Bioengineering·W YuT Yomo
Mar 18, 2006·The Journal of Chemical Physics·M Hase, K Yoshikawa
Aug 8, 2006·Analytical Biochemistry·Takeshi SunamiTetsuya Yomo
Sep 14, 2007·Biochemical and Biophysical Research Communications·Giovanni MurtasPier Luigi Luisi
Sep 15, 2007·Chembiochem : a European Journal of Chemical Biology·Deborah Fiordemondo, Pasquale Stano
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Gerrit van MeerGerald W Feigenson
Sep 2, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Huong Thi BuiRyoichi Kuboi
Nov 26, 2008·Biochimica Et Biophysica Acta·Yutetsu KurumaPier Luigi Luisi
Mar 6, 2009·Chembiochem : a European Journal of Chemical Biology·Tereza Pereira de SouzaPier Luigi Luisi
Jun 18, 2009·Chembiochem : a European Journal of Chemical Biology·Hirohide SaitoDamien Baigl
Oct 20, 2009·Nature·T Martin Schmeing, V Ramakrishnan
Mar 26, 2010·Chembiochem : a European Journal of Chemical Biology·Peter WaldePasquale Stano

❮ Previous
Next ❯

Citations

Jan 18, 2013·PloS One·Kei Fujiwara, Shin-ichiro M Nomura
Jun 7, 2012·The Journal of Chemical Physics·Hiroaki ItoKenichi Yoshikawa
Jul 18, 2014·Current Opinion in Chemical Biology·Norikazu Ichihashi, Tetsuya Yomo
Feb 5, 2013·Current Opinion in Biotechnology·Pasquale Stano, Pier Luigi Luisi
Oct 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·Miho YanagisawaMasayuki Tokita
Oct 4, 2016·ACS Synthetic Biology·Patrick M CaveneyMichael L Simpson
Nov 2, 2017·Physical Biology·Sagardip Majumder, Allen P Liu
Apr 21, 2018·Chembiochem : a European Journal of Chemical Biology·Naoki NakataniKenichi Yoshikawa
Apr 9, 2019·Annals of the New York Academy of Sciences·Norikazu Ichihashi
Apr 3, 2019·Micromachines·Yusuke Sato, Masahiro Takinoue
Jan 13, 2016·Scientific Reports·Yukinori NishigamiMasatoshi Ichikawa
Dec 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Taiki WatanabeShin-Ichiro M Nomura
Jul 21, 2020·Journal of Mass Spectrometry : JMS·Yangjie LiR Graham Cooks
Apr 26, 2015·Bulletin of Mathematical Biology·Fabio MavelliPasquale Stano
Mar 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Emiliano AltamuraFabio Mavelli
Sep 17, 2015·Chembiochem : a European Journal of Chemical Biology·Valentina GalloPier Luigi Luisi
Dec 16, 2018·Bioengineering·Kazusa BeppuRyota Sakamoto
Apr 9, 2020·Frontiers in Bioengineering and Biotechnology·Nadanai LaohakunakornSebastian J Maerkl
May 11, 2018·Scientific Reports·Ryota SakamotoYusuke T Maeda
Apr 3, 2016·Chembiochem : a European Journal of Chemical Biology·Takeshi SunamiTetsuya Yomo
May 21, 2016·Scientific Reports·Bineet SharmaManish Biyani
Mar 25, 2021·ACS Synthetic Biology·By Denis TamievNigel F Reuel
Mar 23, 2021·Biophysics Reviews·Erich Sackmann, Motomu Tanaka
Mar 6, 2021·Annual Review of Biophysics·Thomas Litschel, Petra Schwille
May 9, 2013·ACS Synthetic Biology·Tomoaki MatsuuraTetsuya Yomo
May 10, 2013·ACS Synthetic Biology·Zohreh Nourian, Christophe Danelon
Jan 16, 2020·The Journal of Physical Chemistry. B·Chiho WatanabeMiho Yanagisawa
Dec 23, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Kanae HarusawaMiho Yanagisawa
Nov 11, 2020·ACS Synthetic Biology·Ana I Benítez-MateosFernando López-Gallego
Sep 28, 2021·ACS Nano·Sunidhi C ShettyTom Robinson
Oct 8, 2021·Soft Matter·Eriko ShinoharaMiho Yanagisawa

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.