PMID: 9430238Jan 16, 1998Paper

Cell sorting enriches osteogenic populations in rat bone marrow stromal cell cultures

Bone
A Herbertson, J E Aubin

Abstract

The presence of multiple cell types in bone marrow stromal populations complicates interpretation of cytokine and hormone effects on the osteoprogenitors present, indicating a need for a method for purification of the osteoprogenitor population. Flow cytometric sorting of 7 day primary rat bone marrow stromal cell cultures was performed on the basis of alkaline phosphatase (AP) expression with an antibody against AP (RBM 211.13). The resultant AP(high), AP(low), or control cells were plated to determine osteoprogenitor, macrophage, and adipocyte distribution and frequency. Approximately 50% of osteoprogenitor/bone nodule-forming cells were lost during processing/sorting when compared with unsorted controls. Nevertheless, within the AP(high) fraction, the numbers of AP-positive colonies and osteoprogenitors (bone nodules) were significantly enriched compared with the unfractionated control; the increase in osteoprogenitor frequency ranged from approximately 2 to 100-fold. There were few assayable osteoprogenitors in either the AP(high) or AP(low) fractions in the absence of dexamethasone (dex), suggesting that RBM stroma contains largely dex-dependent osteoprogenitor populations, and that dex may regulate osteoprogenitors subseq...Continue Reading

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Citations

Dec 10, 1999·Cytometry·I L BarbosaA Carvalhais
Jul 17, 2001·Journal of Cellular Biochemistry. Supplement·L Malaval, J E Aubin
Aug 29, 2001·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·C YiY Liu
Aug 21, 2010·Cellular and Molecular Life Sciences : CMLS·Linxia ZhangChristina Chan
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Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·J Bauer
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Jan 20, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S GronthosP J Simmons
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Oct 1, 2005·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Cheolwon SuhJooryung Huh
Jan 1, 2010·Bioinformation·Andre BarkhordarianFrancesco Chiappelli
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Oct 21, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Shinji HiyamaTakashi Uchida
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Apr 3, 2009·Biochemical and Biophysical Research Communications·Chiming YangFabio Rossi
Jul 28, 2016·Stem Cells Translational Medicine·Saad Mechiche AlamiHalima Kerdjoudj
May 7, 2008·Biomedical Materials·Xian-Ying CaoYang Cao

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