PMID: 9538942Apr 16, 1998Paper

Cell-specific differences in the susceptibility of potential cellular targets of human origin derived from blood and lung following treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

Cell Biology and Toxicology
D S LangH S Koren

Abstract

The induction of cytochrome P4501A (CYP1A1) enzyme activity is one of the best-studied direct effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and related compounds and has been shown to be a sensitive biomarker of exposure to polycyclic aromatic hydrocarbons (PAH) in different experimental animal species as well as in humans. TCDD has also been shown to modulate cytokine gene expression in human keratinocytes, including IL-1 beta, TGF-alpha and TFG-beta 2. In the present studies, the aim was to determine whether different cellular targets of human origin differed in susceptibility to TCDD as measured by CYP1A1 activity and mRNA expression, and whether cytokine gene induction/suppression correlated with TCDD susceptibility. Human airway epithelial cells, alveolar macrophages (AM), peripheral blood monocytes and lymphocytes (PBL) were exposed to 10(-10)-10(-7) mol/L TCDD. CYP1A1 enzyme activity was determined by ethoxyresorufin-O-deethylase (EROD) activity, mRNA expression of CYP1A1 was measured by semiquantitative PCR assay. The secretion and/or gene expression of specific cytokines, including IL-6, IL-8, and IL-1 beta were also examined. Overall, there was a clear correlation between TCDD-induced enzyme activity and CYP1A...Continue Reading

Citations

Dec 13, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer L Head WheelerB Paige Lawrence
Mar 29, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sabine TeskeB Paige Lawrence
Nov 17, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Sabine TeskeB Paige Lawrence
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Haley Neff-LaFordB Paige Lawrence
Apr 10, 2010·Systems Biology in Reproductive Medicine·Kaylon L Bruner-Tran, Kevin G Osteen
Apr 18, 2009·American Journal of Respiratory Cell and Molecular Biology·Patrick S WongFumio Matsumura
Jun 23, 2004·Toxicology and Applied Pharmacology·Todd J PageCharles J Czuprynski

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