Feb 1, 1995

Cell-specific expression of fibronectin in adult and developing rabbit lung

Pediatric Research
R A SinkinM B Lomonaco

Abstract

Fibronectin (FN), a glycoprotein component of the extracellular matrix, plays a role in tissue morphogenesis and tissue-specific differentiation through its effects on cell adhesion, cell shape, and cytoskeletal organization. Immunohistochemistry has been used to show that during lung development FN deposition changes, yet the cell-specific sites of pulmonary FN synthesis have not been determined. Because cellular FN synthesis is reflected by FN mRNA abundance, we performed in situ hybridizations to identify pulmonary tissue with the capacity to synthesize FN. Both in situ mRNA hybridization and immunohistochemical staining were performed on tissue sections from lungs of adults and late gestation fetal and neonatal rabbits. In adults, FN transcripts and immunostaining were clearly seen in endothelial cells, smooth muscle cells, and chondrocytes. During lung development, FN transcripts were virtually ubiquitous except in airway epithelium. There was a gradual decrease in FN mRNA abundance with advancing fetal age, but low levels of FN mRNA persisted in neonatal and adult lungs. In contrast, parenchymal immunostaining increased throughout fetal development and remained elevated in the newborn. FN immunostaining was lower in adult...Continue Reading

  • References
  • Citations6

References

  • We're still populating references for this paper, please check back later.

Mentioned in this Paper

Fetal Development Aspects
Immunohistochemistry
Lung
Nucleic Acid Hybridization Procedure
Glycoproteins
Squamous Transitional Epithelial Cell Count
Tissue Specificity
Myocytes, Smooth Muscle
Extracellular Matrix
Cytoskeletal Modeling

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Developmental Dynamics : an Official Publication of the American Association of Anatomists
P L Durham, J M Snyder
Developmental Dynamics : an Official Publication of the American Association of Anatomists
Kimberly A MooreDonald E Ingber
Developmental Dynamics : an Official Publication of the American Association of Anatomists
Seigo MinamiEisuke Mekada
Developmental Dynamics : an Official Publication of the American Association of Anatomists
W V CardosoM C Williams
© 2020 Meta ULC. All rights reserved