Cell-specific localization of glucose transporter proteins in mammalian lung

The Journal of Clinical Endocrinology and Metabolism
S U Devaskar, D E deMello

Abstract

Mammalian lung uses glucose for cellular oxidative metabolism, growth, differentiation, surfactant synthesis, and host defense. Intracellular transport of glucose is accomplished by membrane-associated glycoproteins termed glucose transporters (Gluts). To determine the cell-specific localization patterns, human autopsy lung tissue from preterm (24-32 weeks; n = 4), term infants (38-40 weeks; n = 4), and adults (n = 4) was analyzed for facilitative Glut isoforms and the energy-dependent sodium-glucose cotransporters (SGLT) by Western blot analysis and immunohistochemistry. Antibodies specific for human Glut-1 (erythrocyt, blood-brain barrier type), Glut-3 (brain), Glut-4 (insulin-responsive skeletal muscle/adipocyte), and Glut-5 (kidney/jejunum) were employed. Analysis of Glut-2 (liver/pancreatic beta-cell/small intestine) was performed in newborn and adult rat lungs, and analysis of SGLT1 (kidney/small intestine) was conducted in newborn and adult rabbit lungs, because of the species specificity of the antirat Glut-2 and antirabbit SGLT1 antibodies employed. In human lung at all ages, our studies revealed an approximately 45- to 50-kDa Glut-1 protein band in entrapped erythrocytes and perineural sheaths, which serve as a blood-...Continue Reading

Citations

Feb 2, 2008·Pflügers Archiv : European journal of physiology·Kameljit K KalsiDeborah L Baines
Oct 23, 2012·American Journal of Respiratory Cell and Molecular Biology·Vedang A LondheSherin U Devaskar
Jan 1, 2011·Cancers·Kenneth J O'ByrneSteven G Gray
Jan 11, 2007·American Journal of Physiology. Endocrinology and Metabolism·Amit GangulySherin U Devaskar
Jun 28, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Zhaohui ZhouDaniel P Schuster
Dec 3, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Roberta SüdyBarna Babik

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