Cell-specific pattern of berberine pleiotropic effects on different human cell lines

Scientific Reports
Alessandro AgnarelliSilvia Marracci

Abstract

The natural alkaloid berberine has several pharmacological properties and recently received attention as a potential anticancer agent. In this work, we investigated the molecular mechanisms underlying the anti-tumor effect of berberine on glioblastoma U343 and pancreatic carcinoma MIA PaCa-2 cells. Human dermal fibroblasts (HDF) were used as non-cancer cells. We show that berberine differentially affects cell viability, displaying a higher cytotoxicity on the two cancer cell lines than on HDF. Berberine also affects cell cycle progression, senescence, caspase-3 activity, autophagy and migration in a cell-specific manner. In particular, in HDF it induces cell cycle arrest in G2 and senescence, but not autophagy; in the U343 cells, berberine leads to cell cycle arrest in G2 and induces both senescence and autophagy; in MIA PaCa-2 cells, the alkaloid induces arrest in G1, senescence, autophagy, it increases caspase-3 activity and impairs migration/invasion. As demonstrated by decreased citrate synthase activity, the three cell lines show mitochondrial dysfunction following berberine exposure. Finally, we observed that berberine modulates the expression profile of genes involved in different pathways of tumorigenesis in a cell line...Continue Reading

References

Apr 13, 2004·Oncogene·Devrim Gozuacik, Adi Kimchi
Dec 1, 2005·Molecular Cancer Research : MCR·Cécile-Marie Aliouat-DenisJorge E Vialard
Aug 2, 2007·Nature Reviews. Molecular Cell Biology·Judith Campisi, Fabrizio d'Adda di Fagagna
Jun 10, 2008·Endocrine, Metabolic & Immune Disorders Drug Targets·Jun YinJianping Ye
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jun 2, 2009·Biochemical Pharmacology·Bharat B AggarwalPrashasnika Gehlot
Nov 17, 2010·Genes & Development·Thomas KuilmanDaniel S Peeper
Jun 28, 2011·Biochemical Pharmacology·Annick NotteCarine Michiels
Sep 8, 2012·Advances in Experimental Medicine and Biology·Bilian Jin, Keith D Robertson
Jun 5, 2013·Cold Spring Harbor Perspectives in Biology·Amanda B ParrishSally Kornbluth
Aug 27, 2013·Aging·Hong ZhaoZbigniew Darzynkiewicz
Nov 2, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Bo ShenXin-En Huang
Jul 23, 2014·Molecular Medicine Reports·Yu ZhuBin Hao
Aug 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luis Miguel Guamán OrtizAnna Ivana Scovassi
Dec 18, 2014·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S H ParkN Chung
Jan 8, 2015·Cell Cycle·Véronique Gire, Vjekoslav Dulic
Apr 14, 2015·Seminars in Cancer Biology·Stephanie C CaseyDean W Felsher
Sep 13, 2015·Apoptosis : an International Journal on Programmed Cell Death·Hala Gali-MuhtasibNadine Darwiche
Jun 18, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Zhixiang WangShuang Liang
Mar 8, 2017·Journal of Cellular Physiology·Maryam Moradi BinabajAmir Avan
Mar 14, 2017·The International Journal of Developmental Biology·Linda BalestriniRenata Batistoni
Nov 17, 2017·Clinical and Experimental Pharmacology & Physiology·Dongsheng ShangZhigang Tu

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Citations

Oct 18, 2019·Journal of Cellular and Molecular Medicine·Jiasi WuXianli Meng
Aug 1, 2020·IUBMB Life·Parisa SamadiMehdi Yousefi
Feb 6, 2020·Bioscience, Biotechnology, and Biochemistry·Hinaka YoshidaTomoo Hosoe
Apr 5, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Solomon Habtemariam
Aug 29, 2020·Oxidative Medicine and Cellular Longevity·Maura CalvaniClaudio Favre
Jul 1, 2020·Archives of Virology·Alicja WarowickaAnna Goździcka-Józefiak
Jan 6, 2021·Photodiagnosis and Photodynamic Therapy·Barbara Freitas FlorianoMarília Freitas Calmon
Dec 25, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tairine Zara LopesMarilia Freitas Calmon
Nov 9, 2021·Frontiers in Immunology·Mumin OzturkSuraj P Parihar

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Methods Mentioned

BETA
confocal microscopy
electrophoresis
flow cytometry

Software Mentioned

KC Junior
Excel
Normfinder
BD FACS Software
Statgraphics
Rotor Gene 6000

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