Cell-specific promoter in adenovirus vector for transgenic expression of SERCA1 ATPase in cardiac myocytes

The American Journal of Physiology
Giuseppe InesiC P Ordahl

Abstract

Adenovirus-mediated transfer of cDNA encoding the chicken skeletal muscle sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA1) yielded selective expression in cultured chick embryo cardiac myocytes under control of a segment (-268 base pair) of the cell-specific cardiac troponin T (cTnT) promoter or nonselective expression in myocytes and fibroblasts under control of a constitutive viral [cytomegalovirus (CMV)] promoter. Under optimal conditions nearly all cardiac myocytes in culture were shown to express transgenic SERCA1 ATPase. Expression was targeted to intracellular membranes and was recovered in subcellular fractions with a pattern identical to that of the endogenous SERCA2a ATPase. Relative to control myocytes, transgenic SERCA1 expression increased up to four times the rates of ATP-dependent (and thapsigargin-sensitive) Ca2+ transport activity of cell homogenates. Although the CMV promoter was more active than the cTnT promoter, an upper limit for transgenic expression of functional enzyme was reached under control of either promoter by adjustment of the adenovirus plaque-forming unit titer of infection media. Cytosolic Ca2+ concentration transients and tension development of whole myocytes were also influenced to a simi...Continue Reading

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N Jones, T Shenk
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·E WagnerM L Birnstiel
Jul 1, 1990·Physiological Reviews·G InesiM E Kirtley
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R NagaiM Periasamy
May 1, 1989·Molecular and Cellular Biology·N J KarinD M Fambrough
Dec 1, 1987·Developmental Biology·Z Kaprielian, D M Fambrough
Jan 1, 1988·Methods in Enzymology·M TadaJ Fujii
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A Kass-EislerL A Leinwand
Jul 1, 1993·The Journal of Clinical Investigation·L A KirshenbaumM D Schneider
Oct 1, 1993·Circulation·M D Schneider, B A French
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·J K DonahueJ H Lawrence
Dec 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·M E CARSTEN
Dec 1, 1964·The American Journal of Physiology·G INESIS WATANABE

❮ Previous
Next ❯

Citations

Dec 23, 1999·Pharmacology & Toxicology·J Y Kong, S W Rabkin
Aug 5, 2000·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·C VerdichA Astrup
Nov 1, 2003·American Journal of Physiology. Cell Physiology·Hailun MaGiuseppe Inesi
Jun 5, 2004·Annals of Medicine·Xander H T Wehrens, Andrew R Marks
Mar 8, 2008·Critical Reviews in Toxicology·N Kreke, D R Dietrich
Jan 18, 2006·Digestive Diseases and Sciences·Hermann HarderManfred V Singer
Jul 8, 2005·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·K IwataM Kajimura
Apr 26, 2007·Journal of Cardiovascular Electrophysiology·J Kevin Donahue
Jul 25, 2015·Fish Physiology and Biochemistry·Carla Patrícia Bejo WolkersElisabeth Criscuolo Urbinati
May 27, 2014·Fish Physiology and Biochemistry·Patricia I M SilvaØyvind Øverli
Dec 22, 1999·The American Journal of Physiology·C SumbillaG Inesi
Feb 9, 2007·Muscle & Nerve·Muthu Periasamy, Anuradha Kalyanasundaram
Feb 9, 2007·American Journal of Physiology. Cell Physiology·A M PrasadG Inesi
Mar 13, 2009·European Journal of Gastroenterology & Hepatology·Reawika ChaikominChristopher K Rayner
Jan 14, 1999·The American Journal of Physiology·Y JiM Periasamy
Mar 14, 2021·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Roxanne J SaulnierSimon G Lamarre
Jul 11, 2001·Journal of Molecular and Cellular Cardiology·M Periasamy, S Huke
Jul 2, 2003·Archives of Biochemistry and Biophysics·Carlota SumbillaGiuseppe Inesi
Dec 11, 2007·Biochemical and Biophysical Research Communications·Giuseppe InesiRajendra Pilankatta

❮ Previous
Next ❯

Related Concepts