PMID: 8611634Feb 2, 1996Paper

Cell-specific regulation of the stably expressed serotonin 5-HT1A receptor and altered ganglioside synthesis

Biochimica Et Biophysica Acta
J K SinghP Banerjee

Abstract

Neurotransmission is dependent on the presence of neuronal receptors at the synapses, and important cell surface molecules such as gangliosides are pivotal in the maintenance of synaptic contacts. To study the interrelationship between these two classes of molecules, we achieved stable expression of the hippocampus- and CNS-localized serotonin 1A receptor (5-HT1A-R) in three 5-HT1A-R-deficient neuronal cell lines and also the control, non-neural CHO cells. A strong passage dependence of 5-HT1A-R expression, as measured by mRNA levels as well as membrane binding to the selective agonist [3H]8-OH-DPAT, was observed only in the HN2 (hippocampal) and NCB-20 (CNS) cells which are derived from tissues of natural occurrence of the 5-HT1A-R. A paradigm of stress was obtained by carrying out continuous culture of cells without feeding. During this time a dramatic increase in 5-HT1A-R mRNA and [3H]8-OH-DPAT binding was observed only in the neuronal cells after confluence and during decreased cell viability (days 10/11). This was not due to differentiation, since deliberate serum deprivation and differentiation of cells did not result in any dramatic increase in 5-HT1A-R expression. Analysis of ganglioside synthesis by pulse labeling of t...Continue Reading

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Citations

Mar 28, 2002·Pharmacology & Therapeutics·J R RaymondM N Garnovskaya
Aug 26, 2003·Advanced Drug Delivery Reviews·Lili X Wang, Zaijie Jim Wang
Sep 14, 1999·British Journal of Pharmacology·J R RaymondM N Garnovskaya
Aug 23, 2011·International Journal of Pharmaceutics·Vijaya GopalN Madhusudhana Rao
Apr 3, 2008·Biochimica Et Biophysica Acta·Yamuna Devi PailaAmitabha Chattopadhyay
Jan 30, 2002·The Journal of Biological Chemistry·Lewis R VannSheldon Milstien
May 9, 2000·Journal of Neurochemistry·A García-OstaJ Del Río

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