Cell-specific respiratory activity of aquatic bacteria studied with the tetrazolium reduction method, cyto-clear slides, and image analysis.

Applied and Environmental Microbiology
T PoschR Psenner

Abstract

We present an improvement of the INT [2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride)] reduction method using Cyto-Clear slides, the fluorochrome DAPI (4(prm1),6(prm1)-diamidino-2 phenylindole), and an image analysis system. With this method we were able to simultaneously measure cell dimensions and formazan crystals as indicators of the respiratory activity of single bacteria. The method was tested on a natural bacterioplankton community of an oligotrophic high mountain lake (Gossenkollesee, Tyrolean Alps, Austria, 2,417 m above sea level) in midwinter ((symbl)1-m-thick ice and snow layer; dissolved organic carbon, 0.51 mg liter(sup-1); water temperature, 2(deg)C). About 25% of planktonic bacteria were respiratorily active, and a complex pattern of bacterial morphologies and specific respiratory activities was observed during a time series of INT incubation. Rod-shaped bacteria with cell lengths of between 1.6 and 4.8 (mu)m already showed visible activity after 0.5 h of INT incubation. Small cells (rods and cocci) in the size fraction <1.6 (mu)m and long filamentous bacteria (up to 120 (mu)m) were visibly active only after a 2-h incubation period. After 8 h of incubation, more than 90% of all cells between 3.2...Continue Reading

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Citations

Aug 30, 2001·Applied and Environmental Microbiology·A PernthalerR Amann
Oct 7, 2004·Applied and Environmental Microbiology·Jakob PernthalerKlaus Jürgens
Apr 7, 2004·Applied and Environmental Microbiology·Jonathan PorterRoger W Pickup
May 7, 2003·Applied and Environmental Microbiology·Christine BeardsleyRudolf Amann
Apr 7, 2005·Applied and Environmental Microbiology·Cecilia Alonso, Jakob Pernthaler
Feb 21, 2007·Journal of Applied Microbiology·I WatsonD Stewart-Tull
Oct 31, 1998·Applied and Environmental Microbiology·J PernthalerR Amann
Jul 27, 2002·Annual Review of Microbiology·Angela D Kent, Eric W Triplett
Jan 13, 2005·Letters in Applied Microbiology·K NakajimaM Nasu
Mar 27, 2010·FEMS Microbiology Reviews·Susann Müller, Gerhard Nebe-von-Caron
May 7, 2003·Applied and Environmental Microbiology·Jakob PernthalerRudolf Amann
Sep 13, 2015·FEMS Microbiology Ecology·Tamara Megan Huete-StaufferXosé Anxelu G Morán
Feb 17, 1998·Applied and Environmental Microbiology·M Loferer-KrössbacherR Psenner
Jun 24, 2017·Frontiers in Cellular and Infection Microbiology·Emma Hernandez-SanabriaNico Boon

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