Cell-Specific Transcriptional Responses to Heat Shock in the Mouse Utricle Epithelium

Frontiers in Cellular Neuroscience
Erica SadlerLisa L Cunningham

Abstract

Sensory epithelia of the inner ear contain mechanosensory hair cells (HCs) and glia-like supporting cells (SCs), both of which are required for hearing and balance functions. Each of these cell types has unique responses to ototoxic and cytoprotective stimuli. Non-lethal heat stress in the mammalian utricle induces heat shock proteins (HSPs) and protects against ototoxic drug-induced hair cell death. Induction of HSPs in the utricle demonstrates cell-type specificity at the protein level, with HSP70 induction occurring primarily in SCs, while HSP32 (also known as heme oxygenase 1, HMOX1) is induced primarily in resident macrophages. Neither of these HSPs are robustly induced in HCs, suggesting that HCs may have little capacity for induction of stress-induced protective responses. To determine the transcriptional responses to heat shock of these different cell types, we performed cell-type-specific transcriptional profiling using the RiboTag method, which allows for immunoprecipitation (IP) of actively translating mRNAs from specific cell types. RNA-Seq differential gene expression analyses demonstrated that the RiboTag method identified known cell type-specific markers as well as new markers for HCs and SCs. Gene expression dif...Continue Reading

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Citations

Jul 6, 2021·Journal of Cellular Physiology·Rahul MittalAdrien A Eshraghi
Jun 3, 2021·Trends in Cell Biology·Léo MachadoPhilippos Mourikis

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Methods Mentioned

BETA
RNA-Seq
transgenic
immunoprecipitation
genotyping
chip
PCA
protein folding
FACS

Software Mentioned

GeneCounts
PANTHER Classification System
STAR
Zen
GLAST
REViGO
RiboTag
DESeq2
PCAExplorer
CreER

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