Cell surface 5T4 antigen is transiently upregulated during early human embryonic stem cell differentiation: effect of 5T4 phenotype on neural lineage formation

Experimental Cell Research
Christopher M WardPeter L Stern

Abstract

The 5T4 oncofoetal antigen is a cell surface glycoprotein that is transiently expressed during mouse ES cell differentiation and correlates with decreased pluripotency of such cells. We show that 5T4 antigen is transiently unregulated during HES4 and H1 human ES cell differentiation and its expression correlates with loss of the pluripotent markers OCT-4 and Tra-1-60 and upregulation of transcript markers associated with the three primary germ layers. To confirm that absence of cell surface 5T4 antigen represents a pluripotent hES cell phenotype, we performed mechanical transfer of either 5T4-ve or 5T4+ve HES4 colonies identified using live cell staining. 5T4-ve transfers maintained expression of OCT-4 in over 90% of resultant colonies, whereas 5T4+ve transfers exhibited significantly lower numbers of OCT-4-expressing colonies (92 +/- 1.4 vs. 2.9 +/- 2.0%). Interestingly, low cell density 5T4-ve colony transfers exhibited increased numbers of OCT-4-expressing colonies compared to large 5T4-ve transfers (92 +/- 1.4 vs. 63.2 +/- 1.9%). 5T4-ve and 5T4+ve HES4 and H1 ES cell lines expressed markers representative of neuroectoderm lineages, and we assessed the formation of neural lineages from these phenotypes in serum-containing me...Continue Reading

References

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Citations

Oct 29, 2010·American Journal of Nephrology·Taichi MurakamiToshio Doi
Apr 9, 2008·Stem Cells and Development·Ugur SalliKent E Vrana
Apr 21, 2012·British Journal of Pharmacology·Stuart P AtkinsonLyle Armstrong
Jul 30, 2014·Seminars in Cancer Biology·Peter L SternOwen J McGinn
Oct 21, 2016·Cancer Immunology, Immunotherapy : CII·Peter L Stern, Richard Harrop
Jan 1, 2011·Genes·Francesca Soncin, Christopher M Ward
Dec 4, 2009·Expert Review of Anticancer Therapy·Eyad ElkordRobert E Hawkins
Dec 7, 2007·Cancer Research·Angela M EasthamChristopher M Ward

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