Abstract
beta-1,4-Galactosyltransferase (GalTase) has two functionally distinct subcellular distributions. In the Golgi apparatus, GalTase participates in the glycosylation of secretory and membrane-bound glycoproteins, whereas on the cell surface it mediates specific aspects of intercellular adhesion. For this study, a murine GalTase clone was obtained by screening a lambda gt10 cDNA library made from F9 embryonal carcinoma cells with a heterologous bovine GalTase cDNA probe. The murine GalTase cDNA probe was used in conjunction with assays of GalTase activity to investigate the expression and distribution of GalTase during differentiation of F9 stem cells into secretory endodermal epithelium. During the initial phase of F9 cell differentiation, GalTase mRNA levels remained relatively constant; however, as differentiation progressed, as assayed by expression of the differentiation-specific marker laminin B1, GalTase mRNA levels and enzyme activity rose dramatically. Furthermore, subcellular fractionation of these cells showed that the increased GalTase levels were specifically associated with the Golgi apparatus, whereas GalTase specific activity on the plasma membrane remained constant. These results show that levels of cell surface a...Continue Reading
References
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Solter, B B Knowles
Feb 1, 1976·Nucleic Acids Research·P T Lomedico, G F Saunders
Jan 1, 1985·The Journal of Membrane Biology·A K MircheffG M Gray
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H NarimatsuP K Qasba
Feb 24, 1987·Biochemistry·C A Wilcox, E N Olson
Jan 1, 1986·CRC Critical Reviews in Biochemistry·G J Strous
Apr 8, 1988·Cell·E M BaynaB D Shur
Apr 1, 1988·Journal of Cellular Biochemistry·R B MarchaseM G Humphreys-Beher
Aug 1, 1988·Journal of Biochemistry·K NakazawaH Narimatsu
Aug 1, 1987·Developmental Biology·H K KleinmanG R Martin
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Aug 1, 1973·Archives of Biochemistry and Biophysics·D M CarlsonW J Rutter
Nov 25, 1966·Science·M E Etzler, F Moog
Jan 20, 1981·Biochemistry·M L FrazierG F Saunders
Nov 1, 1983·Gene·U Gubler, B J Hoffman
Aug 15, 1980·Science·G R Martin
Sep 1, 1980·Cell·S StricklandK R Marotti
Jan 24, 1981·Nucleic Acids Research·J MessingP H Seeburg
Jun 1, 1983·Developmental Biology·P C Colony, M R Neutra
Oct 1, 1983·Developmental Biology·B D Shur
Feb 1, 1984·Cell·J CampisiG E Sonenshein
May 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S Tokumitsu, W H Fishman
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C S GasserR T Schimke
Nov 1, 1981·Cell·F HyafilF Jacob
Jan 1, 1981·Histochemistry·S TokumitsuW H Fishman
Citations
Jan 1, 1993·Kidney International·M L TanzerM S Giniger
May 31, 2012·Journal of Cellular Biochemistry·Socrates Villegas-ComonfortEduardo Perez Salazar
Jul 30, 2004·Developmental Biology·Laura de la CruzHelen Hathaway
Mar 20, 1992·Cell·I StamenkovicA Aruffo
Aug 1, 1991·Journal of Neurochemistry·J A RothR Rabin
Nov 24, 1999·Journal of Cellular Physiology·W H MuellerR J Scheibe
Jul 1, 1992·Experimental Cell Research·M H Barcellos-Hoff
Jul 1, 1992·Experimental Cell Research·D J Eckstein, B D Shur
Feb 9, 1996·The Journal of Biological Chemistry·S K ChoR D Cummings
Sep 1, 1995·The Anatomical Record·Y AkimotoH Hirano
Mar 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S C EvansB D Shur
Sep 1, 1991·Somatic Cell and Molecular Genetics·L PounceyV J Kidd
Dec 2, 2005·Oncology·Cristina Vázquez-MartínAlmudena Fernández-Briera
Feb 3, 2018·Oncology Letters·Óscar Mariño-CrespoEmilio Gil-Martín
Nov 1, 1995·Andrologia·E Töpfer-PetersenF Sinowatz
Jul 15, 1990·International Journal of Cancer. Journal International Du Cancer·B AmosR Lotan
Sep 11, 2003·Journal of Cell Science·Helen J HathawayBarry D Shur
May 21, 1998·Mechanisms of Ageing and Development·M E O'BrienA S Weiss
Nov 6, 2002·Biochimica Et Biophysica Acta·Carey Rodeheffer, Barry D Shur
Dec 21, 1993·Biochimica Et Biophysica Acta·R Kleene, E G Berger
Oct 8, 1991·Biochimica Et Biophysica Acta·A S MasibayP K Qasba
Dec 6, 1989·Biochimica Et Biophysica Acta·B D Shur
Oct 1, 1991·Developmental Biology·M B MacekB D Shur