Cell-surface-bound nucleic acids: Free and cell-surface-bound nucleic acids in blood of healthy donors and breast cancer patients

Annals of the New York Academy of Sciences
Pavel P LaktionovValentin V Vlassov

Abstract

Concentrations of extracellular DNA and RNA in the blood of healthy donors and patients with malignant and nonmalignant breast tumors were investigated. Cell-surface-bound extracellular DNA and RNA were detached by PBS-EDTA treatment or mild trypsin treatment of erythrocytes and leukocytes. In healthy donors, almost all extracellular nucleic acids (98%) are bound at the surface of blood cells. In the blood of cancer patients, extracellular nucleic acids were found in plasma and not at the cell surface. In patients with nonmalignant breast tumors, extracellular nucleic acids were found both at the surface of blood cells and in plasma. In healthy donors, the cell-surface-bound DNA is represented by 20-kbp DNA fragments and smaller fragments that varied in amounts in different fractions.

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Citations

Jun 16, 2009·Bulletin of Experimental Biology and Medicine·E S MorozkinP P Laktionov
Jun 16, 2010·Breast Cancer Research and Treatment·Anne M FrielLorraine O'Driscoll
Jun 2, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Tobias A FuchsDenisa D Wagner
Sep 10, 2008·Yi chuan = Hereditas·Ying Hu, Hong Ni
Aug 12, 2014·Bulletin of Experimental Biology and Medicine·K Yu KapitskayaE Yu Rykova
Apr 17, 2012·Expert Opinion on Biological Therapy·Elena Y RykovaPavel P Laktionov
Sep 17, 2011·Biochimica Et Biophysica Acta·Cody C Cook, Masahiro Higuchi
Nov 17, 2006·Annals of the New York Academy of Sciences·Svetlana N TamkovichPavel P Laktionov
Nov 17, 2006·Annals of the New York Academy of Sciences·Elena Y RykovaValentin V Vlassov
Nov 17, 2006·Annals of the New York Academy of Sciences·Olga E BryzgunovaPavel P Laktionov
Nov 17, 2006·Annals of the New York Academy of Sciences·Evgeniy S MorozkinPavel P Laktionov
Oct 8, 2008·Annals of the New York Academy of Sciences·Tatyana E SkvortsovaPavel P Laktionov
Oct 8, 2008·Annals of the New York Academy of Sciences·Peter B Gahan, R Swaminathan
Oct 8, 2008·Annals of the New York Academy of Sciences·Elena V KuliginaVladimir A Richter
Oct 8, 2008·Annals of the New York Academy of Sciences·Vera S Mal'shakovaPavel P Laktionov
Oct 8, 2008·Annals of the New York Academy of Sciences·Svetlana N TamkovichPavel P Laktionov
Oct 8, 2008·Annals of the New York Academy of Sciences·Olga B VainerPavel P Laktionov
Oct 8, 2008·Annals of the New York Academy of Sciences·Elena V KolesnikovaElena Y Rykova
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