Cell-Surface display of heterologous proteins: From high-throughput screening to environmental applications

Biotechnology and Bioengineering
Wilfred Chen, George Georgiou

Abstract

A variety of expression systems for the display of either short peptides or fully folded proteins on E.coli and, to a lesser extent, on Gram-positive bacteria have been developed. The expression of proteins on the surface of microbial cells has proved extremely important for numerous applications ranging from combinatorial library screening and protein engineering, to whole cell biocatalysts and adsorbants for bioremediation purposes.

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Related Concepts

Gram-Positive Bacteria
Gene Expression Regulation, Bacterial
Bacterial Proteins
Combinatorial Chemistry Techniques
Proteins, Recombinant DNA
Metals, Heavy
Gram-Negative Bacteria
In Vitro Molecular Evolution
Microbial
cDNA Library

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