PMID: 7035678Aug 1, 1981Paper

Cell surface distribution of fibronectin in cultures of fibroblasts and bladder derived epithelium: SEM-immunogold localization compared to immunoperoxidase and immunofluorescence

Journal of Microscopy
L K TrejdosiewiczD C Livingston

Abstract

Expression of cell surface fibronectin in cultures of untransformed fibroblasts is well documented, but little is known of its presence and distribution in cultured epithelial cells. Using species monospecific anti-fibronectin antibodies, the distribution of fibronectin in untransformed fibroblasts and in normal and neoplastic bladder epithelial cells was characterized by indirect labelling experiments using immunogold scanning electron microscopy (SEM). The surface matrix of fibronectin expressed in rodent and human fibroblast cell lines was demonstrated with ease by SEM of gold-tagged second antibodies. However, no fibronectin could be detected on any of the mouse and human bladder epithelium-derived cells studied in single or in mixed epithelial-fibroblast cultures. These SEM-immunogold observations were compared to and confirmed by immunofluorescence and immunoperoxidase microscopy. Immunofluorescence and SEM localization of the fibronectin in the extracellular matrix presented similar distribution patterns but the higher resolution of the SEM provided a more detailed analysis.

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Citations

Jun 1, 1985·The Histochemical Journal·R J NichollsL K Trejdosiewicz
Sep 1, 1982·The Histochemical Journal·G M HodgesL K Trejdosiewicz
Jul 1, 1996·Histochemistry and Cell Biology·R HermannM Müller
Jul 1, 1984·The Journal of Cell Biology·E de HarvenH Christensen
Mar 14, 2002·Biochemical and Biophysical Research Communications·Andrew W HorneTimothy A Ryder
Jan 1, 1985·Journal of Microscopy·G ChengL K Trejdosiewicz
May 15, 1984·International Journal of Cancer. Journal International Du Cancer·J K Cowell, L M Franks
May 1, 1988·Journal of Electron Microscopy Technique·A T Sumner
Nov 2, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T GotoD M Brunette
Feb 1, 1986·The American Journal of Anatomy·E de Harven, D Soligo
May 1, 1987·Journal of Microscopy·E de HarvenH Christensen
Feb 1, 1990·The Journal of Urology·H B GrossmanA Flint
Mar 15, 1984·International Journal of Cancer. Journal International Du Cancer·M F Lavin, G J Seymour
Jul 13, 1983·Biochimica Et Biophysica Acta·J G SteeleL M Franks

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