Cell surface-expressed phosphatidylserine and annexin A5 open a novel portal of cell entry.

The Journal of Biological Chemistry
Heidi KenisC Reutelingsperger

Abstract

Expression of phosphatidylserine (PtdSer) at the cell surface is part of the membrane dynamics of apoptosis. Expressed phosphatidylserine functions as an "eat me" flag toward phagocytes. Here, we report that the expressed phosphatidylserine forms part of a hitherto undescribed pinocytic pathway. Annexin A5, a phosphatidylserine-binding protein, binds to and polymerizes through protein-protein interactions on membrane patches expressing phosphatidylserine. The two-dimensional protein network of annexin A5 at the surface prevents apoptotic body formation without interfering with the progression of apoptosis as demonstrated by activation of caspase-3, PtdSer exposure, and DNA fragmentation. The annexin A5 protein network bends the membrane patch nanomechanically into the cell and elicits budding, endocytic vesicle formation, and cytoskeleton-dependent trafficking of the endocytic vesicle. Annexin A1, which binds to PtdSer without forming a two-dimensional protein network, does not induce the formation of endocytic vesicles. This novel pinocytic pathway differs from macropinocytosis, which is preceded by membrane ruffling and actin polymerization. We clearly showed that actin polymerization is not involved in budding and endocytic ...Continue Reading

References

Jan 20, 1991·Journal of Molecular Biology·G MosserA Brisson
Aug 26, 1998·Journal of Structural Biology·I ReviakineA Brisson
Oct 26, 2000·Nature·M O Hengartner
Oct 26, 2000·Nature·J Savill, V Fadok
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·M Leist, M Jäättelä
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Sep 28, 2002·Nature·Marijn G J FordHarvey T McMahon
Oct 12, 2002·Journal of Cell Science·Dorothy I MundyGeorge S Bloom
Dec 3, 2002·International Journal of Radiation Oncology, Biology, Physics·Sophia Ran, Philip E Thorpe
Mar 7, 2003·Nature·Sean D Conner, Sandra L Schmid
Dec 4, 2003·Apoptosis : an International Journal on Programmed Cell Death·S M van den EijndeC Vermeij-Keers
May 1, 1997·Cell Death and Differentiation·S M Van den EijndeC Vermeij-Keers

❮ Previous
Next ❯

Citations

Feb 16, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Francis G BlankenbergMidori Anne Yenari
Jun 7, 2007·European Journal of Nuclear Medicine and Molecular Imaging·S L WoltersL Hofstra
May 5, 2010·Apoptosis : an International Journal on Programmed Cell Death·Kristof Schutters, Chris Reutelingsperger
Aug 11, 2011·Apoptosis : an International Journal on Programmed Cell Death·Sang Youn HwangE K Lee
May 31, 2012·Applied Biochemistry and Biotechnology·Rajiv KumarSudesh Kumar
Sep 20, 2012·Bioconjugate Chemistry·Bryan A Smith, Bradley D Smith
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Dec 13, 2005·Cell Death and Differentiation·B Fadeel, D Xue
Sep 30, 2006·Cell Death and Differentiation·S OuastiM Degli Esposti
Nov 17, 2010·The Journal of Biological Chemistry·Lisette UngethümChris P M Reutelingsperger
Nov 28, 2008·Molecular Biology of the Cell·Mauro Degli EspostiRoya Khosravi-Far
Aug 14, 2009·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Gaku NakatoHiroshi Ohno
Jun 26, 2007·Circulation Journal : Official Journal of the Japanese Circulation Society·Junichi TakiH William Strauss
Jun 24, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Lasse N WaehrensJan T Rasmussen
Nov 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mohammed A S KhanEarl R Stadtman
Oct 14, 2010·Seminars in Immunopathology·Benjamin Frey, Udo S Gaipl
Jun 28, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Howard H ChenDavid E Sosnovik
Mar 25, 2009·Journal of Structural Biology·Nikolay BuzhynskyyAlain R Brisson
Jan 13, 2009·Contrast Media & Molecular Imaging·Geralda A F van TilborgKlaas Nicolay
Jan 28, 2010·Journal of Thrombosis and Haemostasis : JTH·J ZhouG E Gilbert
Mar 15, 2006·Trends in Cell Biology·Yi WuRaymond B Birge
Dec 29, 2005·Experimental Cell Research·Heidi KenisChris P M Reutelingsperger
Dec 6, 2005·Immunobiology·Anna Cederholm, Johan Frostegård
Oct 11, 2007·Differentiation; Research in Biological Diversity·Petr Kaspar, Michal Dvorák
Feb 12, 2008·Protein Expression and Purification·Michael StöckerStefan Barth
Apr 10, 2009·JACC. Cardiovascular Imaging·Jagat Narula, Vasken Dilsizian
Mar 13, 2008·Biochimica Et Biophysica Acta·Hugo O van GenderenChris P M Reutelingsperger
Jun 1, 2014·The American Journal of Cardiology·Jagat Narula, William C Roberts
Feb 26, 2016·Journal of Cellular Physiology·Aaron D Flynn, Hang Yin

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis