Cell-surface Expression of Heat Shock proteins in Dog Neutrophils after Oxidative Stress

Toxicology in Vitro : an International Journal Published in Association with BIBRA
A CaminsP Prieto

Abstract

The effect of oxidative stress induced by different concentrations of hydrogen peroxide on dog neutrophils was studied. This effect was measured using dichlorofluorescein-diacetate (DCFH-DA) and by the cell surface membrane expression of heat shock protein (HSP) 27kDa, HSP 72kDa and HSP 90kDa families. Hydrogen peroxide induced a concentration-dependent increase in DCFH oxidation (from 10(-6)mto 10(-4)m), and an increase in the cell surface expression of HSPs families. At a concentration of 10(-4)m, the percentage of positive cells that showed an oxidation of DCFH was 94.7%+/-5.2 (n=3). Only vitamin E (but not vitamin C) at a concentration of 0.5mm was able to inhibit the intracellular oxidative stress induced by hydrogen peroxide. The percentage of positive cells that express these proteins after the treatment with hydrogen peroxide (10(-4)m) was: 74%+/-3.5 for HSP 27, 72%+/-2.6 for HSP 72 and 73%+/-1.2 for HSP 90 (n=3). This cell surface expression was not abolished by either vitamin C or vitamin E. Localization of HSPs in plasma membrane is of immunological interest because they have been implicated in autoimmune diseases.

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Citations

Apr 27, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Kedsarin Fong-NgernVisith Thongboonkerd
Sep 22, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ilya GukovskyStephen J Pandol
Sep 4, 2021·Cell Stress & Chaperones·Antonio De Maio, Lawrence Hightower

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