PMID: 2105324Feb 1, 1990Paper

Cell surface galactosyltransferase mediates the initiation of neurite outgrowth from PC12 cells on laminin

The Journal of Cell Biology
P C Begovac, B D Shur

Abstract

Neurite outgrowth from PC12 pheochromocytoma cells, as well as from peripheral and central nervous system neurons in vitro, is mediated by the extracellular matrix molecule, laminin. We have recently shown that mesenchymal cell spreading and migration on laminin is mediated, in part, by the cell surface enzyme, beta 1,4 galactosyltransferase (GalTase). GalTase is localized on lamellipodia of migrating cells where it functions as a laminin receptor by binding to specific N-linked oligosaccharides in laminin (Runyan et al., 1988; Eckstein and Shur, 1989). In the present study, we examined whether GalTase functions similarly during neutrite outgrowth on laminin using biochemical and immunological analyses. PC12 neurite outgrowth was inhibited by reagents that perturb cell surface GalTase activity, including anti-GalTase IgG and Fab fragments, as well as the GalTase modifier protein alpha-lactalbumin. Control reagents had no effect on neurite outgrowth. Furthermore, blocking GalTase substrates on laminin matrices by earlier galactosyltion or enzymatic removal of GalTase substrates also inhibited neurite outgrowth. Conversely, neurite outgrowth was enhanced by the addition of UDP-galactose, which completes the GalTase enzymatic reac...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D Schubert, C Whitlock
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Jan 1, 1988·The Journal of Cell Biology·V KünemundM Schachner
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H K KleinmanL Luckenbill-Edds
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J L BixbyL F Reichardt
Oct 14, 1988·Biochemical and Biophysical Research Communications·J W DeanM L Tanzer
Sep 1, 1988·The Journal of Cell Biology·K M NeugebauerL F Reichardt
Sep 4, 1987·Science·R Levi-Montalcini
Oct 1, 1987·Developmental Biology·M Bronner-Fraser
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·A D LanderL F Reichardt
Dec 1, 1983·The Journal of Cell Biology·M ManthorpeS Varon
Jan 1, 1982·Journal of Neuroscience Research·A Baron-Van EvercoorenM E Dubois-Dalcq

❮ Previous
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Citations

Jan 8, 1996·The Journal of Comparative Neurology·A C Puche, B Key
Sep 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T ShirasawaK Hirokawa
Jan 1, 1995·Journal of Cellular Biochemistry·T Y Kostrominova, M L Tanzer
Dec 15, 1993·Journal of Neuroscience Research·S Liang, K A Crutcher
Sep 3, 2008·Science in China. Series C, Life Sciences·Y FanS Zhao
Jun 1, 1991·Developmental Biology·H M Buettner, R N Pittman
Nov 1, 1992·Pharmacology & Therapeutics·V Nurcombe
May 13, 1994·Brain Research. Developmental Brain Research·C AbotomeyC E Hill
Jul 1, 1991·Trends in Pharmacological Sciences·K A Karlsson
Oct 1, 1990·Current Opinion in Cell Biology·B R Zetter, S E Brightman
Oct 1, 1990·Current Opinion in Cell Biology·A D Lander
Oct 1, 1991·Current Opinion in Cell Biology·I de Curtis
Oct 1, 1991·Current Opinion in Neurobiology·K J Tomaselli, K M Neugebauer
Feb 1, 1992·Current Opinion in Neurobiology·J L Bixby
Feb 1, 1997·Brain Research. Brain Research Reviews·L Luckenbill-Edds
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·P A Appeddu, B D Shur
Jan 1, 1991·Annual Review of Neuroscience·L F Reichardt, K J Tomaselli
Dec 1, 1996·Scandinavian Journal of Gastroenterology·M RieglerE Wenzl
Sep 1, 1993·Cell Adhesion and Communication·M GrumetG M Edelman
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·M Paulsson
Aug 1, 1991·Journal of Neuroscience Research·L Gayá-GonzálezJ Lilien
Sep 18, 2012·Biochemical and Biophysical Research Communications·Hee-Jung ChoiKi-Tae Ha
Jan 1, 1993·Kidney International·M L TanzerM S Giniger
Dec 1, 1990·Current Opinion in Cell Biology·H Ploegh, J J Neefjes
Nov 26, 1999·Biochimica Et Biophysica Acta·J S Axford

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