Cell-surface heparan sulfate proteoglycans potentiate chordin antagonism of bone morphogenetic protein signaling and are necessary for cellular uptake of chordin.

The Journal of Biological Chemistry
Reema JasujaDaniel S Greenspan

Abstract

Signaling by bone morphogenetic proteins (BMPs) plays a central role in early embryonic patterning, organogenesis, and homeostasis in a broad range of species. Chordin, an extracellular antagonist of BMP signaling, is thought to readily diffuse in tissues, thus forming gradients of BMP inhibition that result in reciprocal gradients of BMP signaling. The latter determine cell fates along the embryonic dorsoventral axis. The secreted protein Twisted Gastrulation (TSG) is thought to help shape BMP signaling gradients by acting as a cofactor that enhances Chordin inhibition of BMP signaling. Here, we demonstrate that mammalian Chordin binds heparin with an affinity similar to that of factors known to functionally interact with heparan sulfate proteoglycans (HSPGs) in tissues. We further demonstrate that Chordin binding in mouse embryonic tissues was dependent upon its interaction with cell-surface HSPGs and that Chordin bound to cell-surface HSPGs (e.g. syndecans), but not to basement membranes containing the HSPG perlecan. Surprisingly, mammalian TSG did not bind heparin unless prebound to Chordin and/or BMP-4, although Drosophila TSG has been reported to bind heparin on its own. Results are also presented that indicate that Chord...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·G DavidH Van den Berghe
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A J CelesteJ M Wozney
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E A WangP LaPan
Sep 1, 1986·Journal of Cellular Physiology·D Gospodarowicz, J Cheng
Jan 1, 1994·Methods in Enzymology·A C RapraegerB B Olwin
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·A SuzukiN Ueno
Mar 1, 1996·The Journal of Cell Biology·C S Lebakken, A C Rapraeger
Jun 1, 1997·Mechanisms of Development·E D MasonJ L Marsh
Sep 8, 1998·Current Opinion in Genetics & Development·K W Cho, I L Blitz
Oct 6, 1998·Annual Review of Biochemistry·R V Iozzo
Apr 10, 1999·The Journal of Biological Chemistry·S GengrinovitchD Ron
Jun 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P Zimmermann, G David
Apr 15, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·I C ScottD S Greenspan
Jun 29, 2000·Annual Review of Biochemistry·M BernfieldM Zako
Mar 22, 2001·Nature·C ChangA H Brivanlou
Apr 26, 2001·Glycobiology·J Filmus
Nov 29, 2001·The Journal of Cell Biology·B L AllenA C Rapraeger

❮ Previous
Next ❯

Citations

Jun 19, 2013·International Orthopaedics·Araceli Cuellar, A Hari Reddi
Oct 10, 2006·Reviews in Endocrine & Metabolic Disorders·Elisabetta Gazzerro, Ernesto Canalis
Feb 3, 2012·The Journal of Biological Chemistry·Maud ForsbergKarin Forsberg-Nilsson
Aug 12, 2008·Stem Cells and Development·Christian DombrowskiSimon M Cool
Oct 17, 2008·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Mahmud Bani-YaghoubMarianna Sikorska
Sep 15, 2005·BMC Developmental Biology·Giancarlo Ghiselli, Steven A Farber
Oct 27, 2009·Development·David UmulisSeth S Blair
Nov 29, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jean-Louis PlouhinecEdward M De Robertis
Oct 29, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Isao Matsuo, Chiharu Kimura-Yoshida
Nov 4, 2015·Biochemical and Biophysical Research Communications·Alexey M NesterenkoAndrey G Zaraisky
Aug 23, 2008·Journal of Cellular Physiology·Tatsuya MiyazakiOsamu Suzuki
Oct 20, 2007·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Gul San Ara SathiNoriyuki Nagai
Feb 21, 2008·Journal of Biomedical Materials Research. Part a·Marie-Christelle DegatDelphine Logeart-Avramoglou
Dec 29, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Katja JochmannAndrea Vortkamp
Apr 15, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Katja JochmannAndrea Vortkamp
Jun 16, 2010·The Biochemical Journal·Christopher C Rider, Barbara Mulloy
Jul 12, 2008·Developmental Biology·Stephen D FreemanMary E Pownall
May 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chris C Rider, Barbara Mulloy
Jun 11, 2017·Cold Spring Harbor Perspectives in Biology·Joseph ZinskiMary C Mullins
Oct 6, 2006·The Journal of Biological Chemistry·Xiangyang JiaoDavid L Glaser
Jan 27, 2007·The Journal of Biological Chemistry·Reema JasujaDaniel S Greenspan
Apr 4, 2008·American Journal of Physiology. Cell Physiology·Shaukat A KhanPankaj Gupta
May 20, 2006·Biochemical Society Transactions·C C Rider
Jan 21, 2017·The Biochemical Journal·Chandramohan KattamuriThomas B Thompson
Jul 15, 2016·Cold Spring Harbor Perspectives in Biology·Chenbei Chang
Feb 17, 2017·Biochemical Society Transactions·Georg Sedlmeier, Jonathan P Sleeman
Mar 23, 2017·ELife·J Terrig ThomasMalcolm Moos
Aug 5, 2017·JCI Insight·Toshihiro InubushiYu Yamaguchi
Jan 3, 2018·Acta Biochimica Et Biophysica Sinica·Joachim NickelThomas D Mueller
Sep 3, 2020·Experimental Neurology·Christopher G Hart, Soheila Karimi-Abdolrezaee
Oct 20, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Daniel HachimMolly M Stevens
Dec 22, 2005·Development·Michael B O'ConnorSeth S Blair
Oct 18, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Melanie C FisherRobert A Kosher

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.