Cell surface Lactobacillus plantarum LA 318 glyceraldehyde-3-phosphate dehydrogenase (GAPDH) adheres to human colonic mucin

Journal of Applied Microbiology
H KinoshitaTadao Saito

Abstract

To characterize the adhesion molecule of Lactobacillus plantarum LA 318 that shows high adhesion to human colonic mucin (HCM). The adhesion test used the BIACORE assay where PBS-washed bacterial cells showed a significant decrease in adherence to HCM than distilled water-washed cells. A component in the PBS wash fraction adhered to the HCM and a main protein was detected as a c. 40-kDa band using SDS-PAGE. Using homology comparisons of the N-terminal amino acid sequences compared with sequence databases, this protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The DNA sequence of LA 318 GAPDH was 100% identical to the GAPDH (gapB) of L. plantarum WCFS1. The purified GAPDH adhered to HCM. We found the adhesin of L. plantarum LA 318 to HCM in its culture PBS wash fraction. The molecule was identified as GAPDH. Because LA 318 possesses the same adhesin as many pathogens, the lactobacilli GAPDH may compete with pathogens infecting the intestine. This is the first report showing GAPDH expressed on the cell surface of lactobacilli adheres to mucin suggesting L. plantarum LA 318 adheres to HCM using GAPDH binding activity to colonize the human intestinal mucosa.

References

Dec 11, 1992·Science·J KieleczawaF W Studier
Aug 29, 1998·Journal of Applied Microbiology·S AhrnéG Molin
Nov 26, 1999·International Journal of Food Microbiology·M C Durán QuintanaA Garrido Fernández
May 19, 2000·Methods : a Companion to Methods in Enzymology·S S D'Costa, M D Boyle
Oct 29, 2000·Journal of Bacteriology·J SillanpääT K Korhonen
Feb 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michiel KleerebezemRoland J Siezen
Sep 20, 2005·FEMS Microbiology Letters·Mouna OuadghiriJean Swings
Dec 8, 2006·Bioscience, Biotechnology, and Biochemistry·Hideaki UchidaTadao Saito

❮ Previous
Next ❯

Citations

Nov 10, 2009·Applied Microbiology and Biotechnology·Yoshio KatakuraSuteaki Shioya
Jul 5, 2012·The Journal of Biological Chemistry·Tyler J Boone, Gregory J Tyrrell
Feb 2, 2010·Applied and Environmental Microbiology·Ingemar von OssowskiAiri Palva
May 24, 2011·Applied and Environmental Microbiology·Ingemar von OssowskiAiri Palva
Apr 14, 2010·Infection and Immunity·Rachel R Spurbeck, Cindy Grove Arvidson
Aug 31, 2012·Microbial Cell Factories·Gurjot DeepikaDimitris Charalampopoulos
Jun 8, 2012·PloS One·Williams TurpinJean-Pierre Guyot
May 17, 2014·Biology·Veera Kainulainen, Timo K Korhonen
Oct 4, 2014·Advances in Colloid and Interface Science·J BurgainC Gaiani
Dec 29, 2015·Journal of Dairy Science·Yingchun ZhangLinlin Wang
Nov 18, 2011·Trends in Microbiology·Nathalie Juge
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Sep 9, 2009·Journal of Applied Microbiology·D Muñoz-ProvencioV Monedero
Jan 31, 2015·Microbiology·Rebeca MartínLuis G Bermúdez-Humarán
Feb 28, 2015·Journal of Basic Microbiology·Marzia SalzilloRosangela Marasco
Mar 3, 2012·Biochemical and Biophysical Research Communications·Satoru HirayamaYasushi Morinaga
Jun 17, 2009·FEMS Microbiology Letters·Hans Christian BeckAnne Maria Hansen
Jun 26, 2015·PloS One·Vaishak Amblee, Constance J Jeffery

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.