Cell-surface markers for colon adenoma and adenocarcinoma

Oncotarget
Kamini SewdaDavid L Morse

Abstract

Early detection of colorectal cancer (CRC) is crucial for effective treatment. Among CRC screening techniques, optical colonoscopy is widely considered the gold standard. However, it is a costly and invasive procedure with a low rate of compliance. Our long-term goal is to develop molecular imaging agents for the non-invasive detection of CRC by molecular imaging-based colonoscopy using CT, MRI or fluorescence. To achieve this, cell surface targets must be identified and validated. Here, we report the discovery of cell-surface markers that distinguish CRC from surrounding tissues that could be used as molecular imaging targets. Profiling of mRNA expression microarray data from patient tissues including adenoma, adenocarcinoma, and normal gastrointestinal tissues was used to identify potential CRC specific cell-surface markers. Of the identified markers, six were selected for further validation (CLDN1, GPR56, GRM8, LY6G6D/F, SLCO1B3 and TLR4). Protein expression was confirmed by immunohistochemistry of patient tissues. Except for SLCO1B3, diffuse and low expression was observed for each marker in normal colon tissues. The three markers with the greatest protein overexpression were CLDN1, LY6G6D/F and TLR4, where at least one of ...Continue Reading

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Citations

Aug 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Monica I AlvarezDennis C Ko
Jan 27, 2018·Oncotarget·Allison S CohenDavid L Morse
Sep 27, 2019·Protein and Peptide Letters·Ruipu SunRen Lai
Sep 27, 2018·Molecular & Cellular Oncology·Michelle H TownsendKim L O'Neill
Mar 17, 2020·Cellular and Molecular Bioengineering·Mahboubeh S NooriDouglas J Goetz
May 17, 2017·Expert Opinion on Emerging Drugs·Abdallah Abou Zahr, Gautam Borthakur
Apr 11, 2021·The Journal of Biological Chemistry·Treena ChatterjeeKendra S Carmon
Dec 17, 2020·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Hong-Tao HuSuhwan Chang
Apr 8, 2020·ACS Pharmacology & Translational Science·Abanoub A Gad, Nariman Balenga
Dec 14, 2021·Frontiers in Cell and Developmental Biology·Xuepu SunHaiquan Qiao

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Methods Mentioned

BETA
biopsy
electrophoresis
PCR
scraping
protein assay
X-ray

Software Mentioned

Expression Console
GraphPad Prism
GeneMarker

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