Cell Surface Mechanics Gate Embryonic Stem Cell Differentiation.

Cell Stem Cell
Martin BergertAlba Diz-Munoz

Abstract

Cell differentiation typically occurs with concomitant shape transitions to enable specialized functions. To adopt a different shape, cells need to change the mechanical properties of their surface. However, whether cell surface mechanics control the process of differentiation has been relatively unexplored. Here we show that membrane mechanics gate exit from naive pluripotency of mouse embryonic stem cells. By measuring membrane tension during early differentiation, we find that naive stem cells release their plasma membrane from the underlying actin cortex when transitioning to a primed state. By mechanically tethering the plasma membrane to the cortex by enhancing Ezrin activity or expressing a synthetic signaling-inert linker, we demonstrate that preventing this detachment forces stem cells to retain their naive pluripotent identity. We thus identify a decrease in membrane-to-cortex attachment as a new cell-intrinsic mechanism that is essential for stem cells to exit pluripotency.

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Citations

Nov 21, 2020·Cell Stem Cell·Henry De BellyKevin J Chalut
Feb 6, 2021·Cell Stem Cell·Jonathon M Muncie, Valerie M Weaver
Mar 2, 2021·Frontiers in Cell and Developmental Biology·Galym IsmagulovGuojun Sheng
Dec 15, 2020·Developmental Cell·Erik S WelfGaudenz Danuser
Apr 1, 2021·Biology of the Cell·Umidahan DjakbarovaCömert Kural
Jun 3, 2021·Life·Andrea CugnoKlaus Ley
Jun 10, 2021·Developmental Cell·Mary-Kate HaywardValerie M Weaver

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Datasets Mentioned

BETA
E-MTAB-9404

Methods Mentioned

BETA
scanning electron microscopy
atomic force spectroscopy
atomic force microscopy
lipidation
RNA-seq
scanning electron
flow cytometry

Software Mentioned

enrichR
limma
JPK Data Processing
edgeR
STAR
FlowJo

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