PMID: 3771645Oct 1, 1986Paper

Cell surface modulation of the neural cell adhesion molecule resulting from alternative mRNA splicing in a tissue-specific developmental sequence

The Journal of Cell Biology
B A MurrayG M Edelman

Abstract

The neural cell adhesion molecule N-CAM is an intrinsic membrane glycoprotein that is expressed in the embryonic chicken nervous system as two different polypeptide chains encoded by alternatively spliced transcripts of a single gene. Because they differ by the presence or absence of approximately 250 amino acids in their cytoplasmic domains, these polypeptides are designated ld and sd, for large and small cytoplasmic domain, respectively. We report here that the ld-specific sequences comprise a single exon in the chicken N-CAM gene and that developmental expression of the ld and sd chains occurs in a tissue-specific fashion, with the ld chain restricted to the nervous system. Comparison of the nucleotide sequences from an N-CAM genomic clone with cDNA sequences showed that a single exon of 783 base pairs corresponded to the unique cytoplasmic domain of the ld polypeptide. Sequences from this exon were absent from the single N-CAM mRNA detected in several non-neural tissues by RNA blot hybridization, and immunoblot analysis confirmed that antigenic determinants unique to the ld-specific domain were not expressed in these tissues. Immunohistochemical experiments indicated that only the sd chain was expressed on cell surfaces of ...Continue Reading

References

Aug 22, 1985·Nature·M NobleU Rutishauser
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J J HemperlyB A Cunningham
Feb 1, 1986·The Journal of Cell Biology·D R FriedlanderG M Edelman
Jul 1, 1986·The Journal of Cell Biology·S HoffmanG M Edelman
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J E SchwarzbauerR O Hynes
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E OdermattR O Hynes
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K L CrossinG M Edelman
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P D'EustachioB A Cunningham
Jul 1, 1985·The Journal of Cell Biology·F RiegerG M Edelman
Jan 1, 1985·Annual Review of Biochemistry·G M Edelman
Sep 1, 1985·The Journal of Cell Biology·C M Chuong, G M Edelman
Nov 1, 1985·Experimental Cell Research·G M Edelman
Mar 1, 1986·The Journal of Cell Biology·J CovaultJ R Sanes
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B A MurrayB A Cunningham
Nov 15, 1983·Journal of Molecular Biology·A M FrischaufN Murray
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M E GreenbergG M Edelman
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B A CunninghamG M Edelman
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G M EdelmanJ P Thiery
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Edelman
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G M Edelman, C M Chuong
Jan 22, 1982·Nucleic Acids Research·S M Mount
Aug 21, 1981·Science·W E Gall, G M Edelman

❮ Previous
Next ❯

Citations

Apr 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N EndoA Schmidt
Jul 1, 1993·Clinical & Experimental Metastasis·S M Brady-KalnayR Brackenbury
Jan 1, 1990·Journal of Molecular Neuroscience : MN·J J HemperlyR A Reid
Jun 19, 1992·Brain Research. Developmental Brain Research·I WallisK H Pfenninger
Jan 1, 1992·Trends in Neurosciences·J W Fawcett
Jul 1, 1989·Brain Research. Molecular Brain Research·P Ramos, L Ellis
Jan 1, 1988·Neurochemistry International·O NybroeE Bock
Oct 11, 2003·Biochemical and Biophysical Research Communications·Bettina BüttnerRüdiger Horstkorte
Jan 21, 2000·Brain Research. Molecular Brain Research·C S RicardM R Hernandez
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J J HemperlyB A Cunningham
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G C OwensB A Cunningham
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G M EdelmanW J Gallin
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E A PredigerB A Cunningham
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K J GreenR L Chisholm
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A L PrietoG M Edelman
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S LiM G Rosenfeld
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·T S RanheimB A Cunningham
Apr 1, 1993·The European Journal of Neuroscience·E S WintergerstU Bartsch
Mar 3, 2012·PloS One·Marie-Claude AmoureuxGeneviève Rougon
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·E B LittleB A Cunningham
Jul 5, 2008·Neurochemical Research·Kateryna Kolkova
Sep 1, 1993·Neuromuscular Disorders : NMD·C Cifuentes-DiazR M Mège
Apr 1, 1991·American Journal of Obstetrics and Gynecology·B A Cunningham
Aug 1, 1994·Teratology·S A Neale, D G Trasler
Mar 3, 2004·Journal of Neuroscience Research·Bettina BüttnerRüdiger Horstkorte
Apr 24, 2008·Progress in Neuro-psychopharmacology & Biological Psychiatry·Yusuke WakabayashiYoshifumi Watanabe
Sep 24, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Tania RoskamsLouis Libbrecht
Jun 1, 1994·Critical Reviews in Oncology/hematology·C VerfaillieJ B McCarthy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.