Cell-surface processing of pro-ADAMTS9 by furin

The Journal of Biological Chemistry
Bon-Hun KooSuneel S Apte

Abstract

Processing of polypeptide precursors by proprotein convertases (PCs) such as furin typically occurs within the trans-Golgi network. Here, we show in a variety of cell types that the propeptide of ADAMTS9 is not excised intracellularly. Pulse-chase analysis in HEK293F cells indicated that the intact zymogen was secreted to the cell surface and was subsequently processed there before release into the medium. The processing occurred via a furin-dependent mechanism as shown using PC inhibitors, lack of processing in furin-deficient cells, and rescue by furin in these cells. Moreover, down-regulation of furin by small interference RNA reduced ADAMTS9 processing in HEK293F cells. PC5A could also process pro-ADAMTS9, but similarly to furin, processed forms were absent intracellularly. Cell-surface, furin-dependent processing of pro-ADAMTS9 creates a precedent for extracellular maturation of endogenously produced secreted proproteins. It also indicates the existence of a variety of mechanisms for processing of ADAMTS proteases.

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K R KlimpelS H Leppla
Sep 15, 1993·Biochemical and Biophysical Research Communications·G VidricaireR Leduc
Sep 25, 1998·The Journal of Biological Chemistry·L LumC P Blobel
Jan 28, 1999·The Journal of Biological Chemistry·M RoghaniC P Blobel
Jun 22, 1999·The Journal of Biological Chemistry·K KunoK Matsushima
Jul 20, 1999·The Journal of Biological Chemistry·A ZhouD F Steiner
Oct 6, 1999·Current Opinion in Cell Biology·G Murphy, J Gavrilovic
Feb 5, 2000·Journal of Molecular Endocrinology·F BergeronR Day
Jul 11, 2000·Molecular Biology of the Cell·I Yana, S J Weiss
Aug 17, 2000·The Journal of Biological Chemistry·J C Rodriguez-ManzanequeM L Iruela-Arispe
Jun 16, 2001·The Journal of Biological Chemistry·R J FernandesS S Apte
May 10, 2002·The Journal of Biological Chemistry·Yi CaoAnna Zolkiewska
May 22, 2002·Current Opinion in Pharmacology·Elizabeth C Arner
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Gary Thomas
Dec 5, 2002·Essays in Biochemistry·Nabil G Seidah, Annik Prat
Jan 7, 2003·The Journal of Biological Chemistry·Robert P T SomervilleSuneel S Apte
Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Suneel S Apte
Apr 22, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Gaétan MayerMoïse Bendayan
Jun 9, 2004·The Journal of Biological Chemistry·Jean-Michel Longpré, Richard Leduc
Jun 12, 2004·The Journal of Biological Chemistry·Robert P T SomervilleSuneel S Apte
Sep 10, 2004·The Journal of Biological Chemistry·Robert P T SomervilleSuneel S Apte
Sep 16, 2004·American Journal of Human Genetics·Nathalie DagoneauValérie Cormier-Daire
May 19, 2005·The International Journal of Biochemistry & Cell Biology·Julie Huxley-JonesRaymond P Boot-Handford
Jun 9, 2005·Gene Expression Patterns : GEP·Katherine A JungersSuneel S Apte

❮ Previous
Next ❯

Citations

Nov 15, 2012·The Journal of Biological Chemistry·Anna J S HoubenWouter H Moolenaar
Nov 28, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Véronique ThimonJean-Luc Gatti
Dec 7, 2013·American Journal of Respiratory Cell and Molecular Biology·Ahmed LazrakSadis Matalon
Jan 24, 2012·Best Practice & Research. Clinical Rheumatology·Amanda J Fosang, Frank Beier
Feb 27, 2007·Biochemical and Biophysical Research Communications·Sang-Nam LeeIris Lindberg
Nov 11, 2008·The International Journal of Biochemistry & Cell Biology·Jean-Michel LongpréRichard Leduc
Jul 22, 2008·The FEBS Journal·Shinji Ihara, Kiyoji Nishiwaki
Mar 17, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Johanne Dubail, Suneel S Apte
Jun 21, 2011·Matrix Biology : Journal of the International Society for Matrix Biology·Christopher Kintakas, Daniel R McCulloch
Apr 21, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Ursula R RodgersIan M Clark
Jan 26, 2010·Matrix Biology : Journal of the International Society for Matrix Biology·Christine B KernSuneel S Apte
Dec 26, 2016·Journal of Biochemistry·Tsutomu NakagawaFumiaki Suzuki
Aug 19, 2016·Scientific Reports·Go-Woon KimKyung-Ok Cho
Jan 1, 2012·Cancers·Saran KumarRuowen Ge
Oct 1, 2016·Scientific Reports·Johanne DubailSuneel S Apte
Apr 9, 2011·Clinical Chemistry·Alexander G SemenovAlexey G Katrukha
Apr 4, 2007·The Journal of Biological Chemistry·Bon-Hun KooSuneel S Apte
Oct 31, 2009·The Journal of Biological Chemistry·Bon-Hun Koo, Suneel S Apte
Apr 16, 2020·Critical Reviews in Biochemistry and Molecular Biology·Lise BoonGhislain Opdenakker
Aug 2, 2008·Archives of Biochemistry and Biophysics·Anne-Marie MalfaitMicky D Tortorella

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Archives of Biochemistry and Biophysics
Micky D TortorellaAnne-Marie Malfait
Archives of Biochemistry and Biophysics
Anne-Marie MalfaitMicky D Tortorella
© 2021 Meta ULC. All rights reserved