Cell surface receptor-specific scaffold requirements for adhesion to laminin-derived peptide-chitosan membranes

Biomaterials
Kentaro HozumiM Nomizu

Abstract

Scaffolds are used for bioengineering to regulate cellular functions. Previously, we developed laminin-derived peptide-conjugated chitosan membranes for cell engineering. Here, we determined whether changes in the chitosan scaffold altered the cellular response. When an alphavbeta3 integrin-binding peptide A99a (ALRGDN) was conjugated on chitosan membranes of varying density (1.5-1500 ng/mm(2)), cell adhesion was altered depending on the amount of chitosan. 3 or 30 ng/mm(2) of the A99a-chitosan membrane effectively promoted cell attachment, cell spreading with well-organized actin stress fibers, phosphorylation of FAK Tyr397, and neurite outgrowth. In contrast, syndecan-binding peptide AG73 (RKRLQVQLSIRT) conjugated chitosan membranes density (1.5-1500 ng/mm(2)) promoted similar biological activities at all of the concentrations tested. These results suggest that integrin-mediated cell adhesion is sensitive to the scaffold condition. To improve the function of integrin-mediated biological activities on a large amount of scaffold, we designed an A99a/AG73 mixed peptide-chitosan membrane. The mixed peptide-chitosan membrane promoted the strongest biological activities at 150-1500 ng/mm(2) of chitosan membrane. We conclude that th...Continue Reading

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Feb 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Timpl, J C Brown
Nov 26, 1998·The Journal of Biological Chemistry·M NomizuY Yamada
Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Colognato, P D Yurchenco
Jul 20, 2002·The Journal of Biological Chemistry·Ikuko OkazakiMotoyoshi Nomizu
Nov 26, 2002·The Journal of Cell Biology·Catherine G GalbraithMichael P Sheetz
Mar 11, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mayumi MochizukiMotoyoshi Nomizu
Sep 17, 2003·The Journal of Biological Chemistry·Kyle J McQuade, Alan C Rapraeger
Feb 13, 2004·The Journal of Biological Chemistry·Akira KatsumiMartin Alexander Schwartz
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Jeffrey H Miner, Peter D Yurchenco
Jun 25, 2005·Seminars in Cancer Biology·Hynda K Kleinman, George R Martin
Nov 26, 2005·The Anatomical Record. Part B, the New Anatomist·Stephen F Badylak
Jul 28, 2006·Journal of Biomedical Materials Research. Part a·Shigehiro IkemotoYuichi Kadoya
Jan 20, 2007·Biopolymers·Mayumi MochizukiMotoyoshi Nomizu
May 26, 2007·Biomaterials·Stephen F Badylak
Oct 30, 2007·Journal of Pharmaceutical Sciences·Joshua S BoatengGillian M Eccleston
Jan 1, 2008·Journal of Materials Science. Materials in Medicine·Galo CárdenasJackeline Sepúlveda
Apr 9, 2008·Current Topics in Medicinal Chemistry·Tao JiangCato T Laurencin
Oct 22, 2008·Acta Biomaterialia·Stephen F BadylakThomas W Gilbert
Jan 21, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ryuji MasudaYuichi Kadoya
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Benjamin GeigerAlexander D Bershadsky

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Citations

Jun 2, 2012·The Journal of Biological Chemistry·Kentaro HozumiMotoyoshi Nomizu
Nov 16, 2012·Science Translational Medicine·E Thomas Pashuck, Molly M Stevens
Jul 6, 2010·FEBS Letters·Kentaro HozumiMotoyoshi Nomizu
Oct 7, 2011·Journal of Biomedical Materials Research. Part a·Yamato KikkawaMotoyoshi Nomizu
Oct 10, 2013·Advanced Healthcare Materials·Takashi HoshibaMasaru Tanaka
Dec 25, 2012·Cell Adhesion & Migration·Yamato KikkawaJennifer E Koblinski
Jun 15, 2013·Biomaterials·Yuji YamadaMotoyoshi Nomizu
Dec 25, 2015·Biotechnology Progress·Abdulhadi AljawishIsabelle Chevalot
Mar 31, 2017·Journal of Biomaterials Science. Polymer Edition·Takashi HoshibaMasaru Tanaka
Jan 10, 2018·Advanced Healthcare Materials·Daniel A FoytEileen Gentleman
Sep 14, 2018·International Journal of Molecular Sciences·Kentaro Hozumi, Motoyoshi Nomizu
Dec 29, 2020·Experimental Cell Research·Hideki HayashiMotoyoshi Nomizu
Apr 22, 2018·Archives of Biochemistry and Biophysics·Hideki HayashiMotoyoshi Nomizu
Mar 17, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Takashi HoshibaKeita Sakakibara

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