Cell survival and proliferation in Drosophila S2 cells following apoptotic stress in the absence of the APAF-1 homolog, ARK, or downstream caspases

Apoptosis : an International Journal on Programmed Cell Death
S Kiessling, D R Green

Abstract

In Drosophila, the APAF-1 homolog ARK is required for the activation of the initiator caspase DRONC, which in turn cleaves the effector caspases DRICE and DCP-1. While the function of ARK is important in stress-induced apoptosis in Drosophila S2 cells, as its removal completely suppresses cell death, the decision to undergo apoptosis appears to be regulated at the level of caspase activation, which is controlled by the IAP proteins, particularly DIAP1. Here, we further dissect the apoptotic pathways induced in Drosophila S2 cells in response to stressors and in response to knock-down of DIAP1. We found that the induction of apoptosis was dependent in each case on expression of ARK and DRONC and surviving cells continued to proliferate. We noted a difference in the effects of silencing the executioner caspases DCP-1 and DRICE; knock-down of either or both of these had dramatic effects to sustain cell survival following depletion of DIAP1, but had only minor effects following cellular stress. Our results suggest that the executioner caspases are essential for death following DIAP1 knock-down, indicating that the initiator caspase DRONC may lack executioner functions. The apparent absence of mitochondrial outer membrane permeabili...Continue Reading

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Citations

Jul 18, 2006·Annual Review of Cell and Developmental Biology·Bruce A Hay, Ming Guo
Dec 19, 2015·Apoptosis : an International Journal on Programmed Cell Death·Amandine ClavierIsabelle Guénal
Sep 30, 2014·Genome Biology·Hangnoh LeeDavid MacAlpine
Mar 8, 2007·Journal of Cell Science·David A PrimroseEdan Foley

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