PMID: 8997666Jan 1, 1997Paper

Cell swelling activates the K+ conductance and inhibits the Cl- conductance of the basolateral membrane of cells from a leaky epithelium

The Journal of General Physiology
R J TorresL Reuss

Abstract

Necturus gallbladder epithelial cells bathed in 10 mM HCO3/1% CO2 display sizable basolateral membrane conductances for Cl- (GClb) and K+ (GKb). Lowering the osmolality of the apical bathing solution hyperpolarized both apical and basolateral membranes and increased the K+/Cl- selectivity of the basolateral membrane. Hyperosmotic solutions had the opposite effects. Intracellular free-calcium concentration ([Ca2+]i) increased transiently during hyposmotic swelling (peak at approximately 30 s, return to baseline within approximately 90 s), but chelation of cell Ca2+ did not prevent the membrane hyperpolarization elicited by the hyposmotic solution. Cable analysis experiments showed that the electrical resistance of the basolateral membrane decreased during hyposmotic swelling and increased during hyperosmotic shrinkage, whereas the apical membrane resistance was unchanged in hyposmotic solution and decreased in hyperosmotic solution. We assessed changes in cell volume in the epithelium by measuring changes in the intracellular concentration of an impermeant cation (tetramethylammonium), and in isolated polarized cells measuring changes in intracellular calcein fluorescence, and observed that these epithelial cells do not undergo ...Continue Reading

References

Jul 1, 1992·The American Journal of Physiology·J L Garvin, K R Spring
Feb 1, 1992·The Journal of General Physiology·G A AltenbergL Reuss
Jun 1, 1990·The American Journal of Physiology·T J Furlong, K R Spring
Jan 1, 1991·Annual Review of Physiology·L ReussG Altenberg
Sep 1, 1989·The American Journal of Physiology·J S Stoddard, L Reuss
Apr 1, 1989·Physiological Reviews·L Reuss
Jan 1, 1985·The American Journal of Physiology·K U Petersen, L Reuss
May 1, 1988·The Journal of Membrane Biology·J S Stoddard, L Reuss
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L Reuss
May 1, 1983·The Journal of General Physiology·K U Petersen, L Reuss
Jan 1, 1984·The Journal of Membrane Biology·M Larson, K R Spring
Jan 1, 1983·The Journal of Membrane Biology·A Corcia, W M Armstrong
Mar 1, 1982·The Journal of General Physiology·B E Persson, K R Spring
Jun 1, 1994·The Journal of Membrane Biology·D Chesnoy-Marchais, J Fritsch
Jul 1, 1994·The American Journal of Physiology·M A Robertson, J K Foskett
Feb 1, 1994·The American Journal of Physiology·R Dausch, K R Spring
Jan 1, 1994·The Journal of General Physiology·T A HemingL Reuss
Jan 1, 1994·Methods in Cell Biology·J P Kao
Dec 1, 1993·The American Journal of Physiology·G A AltenbergL Reuss
Jun 1, 1996·The American Journal of Physiology·R J TorresL Reuss
Nov 1, 1996·The American Journal of Physiology·R J TorresL Reuss

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Citations

Sep 28, 2000·Biochemical and Biophysical Research Communications·G MeyerG Bottà
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·C G Vanoye, L Reuss
Apr 6, 2007·Journal of Theoretical Biology·Kazumoto IguchiHiroaki S Yamada
Feb 10, 1999·The American Journal of Physiology·C G VanoyeG A Altenberg
Oct 12, 1999·The American Journal of Physiology·V LyallG M Feldman
May 8, 1999·The American Journal of Physiology·T J BirdseyS L Greenwood

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